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《Central South Pharmacy》 2009-07
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Determination of puerarin in rat plasma after intragastric administration of puerarin microemulsion

DENG Chang-feng1,2,GONG You-lan1,2,WU Xue1,2,XIANG Da-wei1,2,OU-YANG Li-na1,2,XIANG Da-xiong1,2(1.School of Pharmaceutical Sciences,Central South University,Changsha 410013;2.Department of Pharmacy,Second Xiangya Hospital,Central South University,Changsha 410011)  
Objective To establish an RP-HPLC method for the determination of puerarin in rat plasma to be applied in the bioavailability study of puerarin microemulsion in rats.MethodsPlasma samples were treated with acetonitrile to deposit proteins,and then were analyzed by HPLC on a Phenomenex C18 column(250 mm×4.6 mm,5 μm) using acetonitrile-0.1% acetic acid solution(23∶77)mixture solvent as the mobile phase at 0.8 mL·min-1.The column effluent was monitored by UV detector at 250 nm and 4-hydroxy-benzaldehyde was used as the internal standard.The column temperature was 30 ℃,and the injection volume was 20 μL.ResultsThe calibration curve of puerarin in rat plasma at 0.10~10.00 μg·mL-1 was excellently linear(r=0.999 6),with the lower limit of quantification of 0.10 μg·mL-1.The extraction recoveries were larger than 79%,the method recoveries larger than 92%,and the intra-and inter-day RSD smaller than 6%.ConclusionThe validated method is sensitive,accurate and suitable for the determination of puerarin in rat plasma and bioavailability study of puerarin microemulsion in rats.
【Fund】: 湖南省自然科学基金项目(No.08JJ3053)
【CateGory Index】: R285
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