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《Central South Pharmacy》 2010-08
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Preparation of molecular imprinted polymer of neferine

LIU Shao1,2,3,LI Xin-zhong1,2,LEI Peng1,2,HONG Li-xia2(1.Department of Pharmacy,Xiangya Hospital,Central South University,Changsha 41008;2.School of Pharmacy,Central South University,Changsha 410083;3.Research Center of Modernization of Chinese Medicines,Central South University,Changsha 410083)  
Objective To prepare molecular imprinted polymers(MIPs)of neferine.Methods MIPs of neferine were synthesized by hot-initiated polymerization with neferine as imprinting molecules,a-methacrylic acid(a-MAA) as the functional monomer and ethylene glycol dimethacrylate(EDMA) as the cross-linker.Uniform design was used to optimize the condition.The selectivity of the prepared MIP of neferine was studied by packing it in a solid-phase extraction column.Results The optimum preparation condition was as follows:neferine 0.1 mmol,temperature 50 ℃,a-MAA 0.8 mmol,and EDMA 2.5 mmol.Conclusion The MIP prepared by optimum method exhibits high selectivity and good recognition ability to neferine.
【Fund】: 国家高新技术研究发展计划(863计划)(编号:2007AA021802)
【CateGory Index】: TQ461
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