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《Journal of Chongqing Medical University》 2003-04
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Methodology of separation, purification, cultivation and identification of rat bone marrow macrophage

LI Jing,et al (Department of Histology and Embryology, College of Basic Medical Sciences, Chongqing Medical University)  
Objective:To study the biological functions of bone marrow macrophage (BMM Φ) and to establish the methodology of separation, purification, cultivation and identification of BMM Φ.Methods:Using the techniques of anchorage-dependent culture of separated rat bone marrow cell (rBMC) in DMEM culture media (contain 20% horse serum,20% (v/v)L 929 conditioned media) in vitro,a lot of purified anchor cells were obtained, and these cells were identified with specifically biological marker of macrophage, such as 1, morphologicalobservation:invert phase contrast microscopy, light and electron microscopy;2, enzyme cytochemistry:acid phosphatase (ACP),α-acetic acid naphthol esterase (α-ANE);3,phagocytic experiment:phagocytosis of chicken erythrocytes and prepared Chinese ink;4,immunocytochemistry: surface specific antigen of macrophage ( CD 68 stain).Results:The cells were purified having functional satisfactory macrophage according to morphological observation,enzyme cytochemistry, phagocytic experiment and immunocytochemistry.Conclusion:This is a simple and easy method for separation, purification, cultivation and identification of rat marrow macrophage.
【CateGory Index】: R329.2
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