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《Journal of Chongqing Medical University》 2004-04
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Construction and expression of the pIRES-enchanced green fluorescent protein reporter gene vector carrying BMP_2 Gene

ZOU Haibo,et al (Department of Orthopaedic Surgery,College of Clinical Medicine,Chongqing Medical University)  
Objective:To construct the pIRES2-enchanced green flurescent protein expression vector carrying BMP_2 gene in order to provide an ideal reporter gene for the expression and identify the location of portein in vitro and vivo.Methods:The BMP_2 cDNA sequence was excised,and subcloned on pUC18 vector.And then the BMP_2 sequence on the pUC18 plasmid was excised again and ligated into Sal Ⅰ site of pIRES2-EGFP treated by phosphorylase in sense and antisense direction.The correct recombinant plasmid was transfected into 293-T cells,the expression of BMP_2 was determined by FCM,LCM and RT-PCR.Results:The recombinant expression vector was verified correctly by enzyme digestion,sequence analysis.This vector was expressed in cells.Conclusion:A pIRES2 green fluorescent protein reporter gene vector containing BMP_2 has been constructed successfully,thus providing an important and convenient tool to study intracellular localization of BMP_2.
【CateGory Index】: R346
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【References】
Chinese Journal Full-text Database 3 Hits
1 XU Zhong-ye1,CHENG Yuan1,ZHU Xiao-feng2,et al.(1.Department of Neurosurgery,Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010,China;2.Neuro Science Research Institul Jiamusi University,Jianmusi 154003,China;3.Department of Neuro Surgery,First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China);Construct and identification of the pIRES2-enchanced green flurescent protein expression vector carrying hBDNF gene[J];Chongqing Medicine;2008-10
2 Ding Xing-po1, Jin Xian-qing2, Wang Zhi-yong1, Fu Yan-li11Research Laboratory of Pediatric Ortho- paedics, Henan Academy of Ortho- paedics, Luoyang 471002, He'nan Province, China; 2Research Institute of Pediatrics, Chongqing Medical University, Chongqing 400014, China;Transfection of human insulin-like growth factor-1 gene into rabbit mesenchymal stem cells mediated by nanometer vectors and the gene expression[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2008-06
3 LI Xingsheng,et al the Institute of Ultrasound Imaging,Chongqing Medical University;Construction and expression of enhanced green fluorescent protein and HGF co-expression vector[J];Journal of Chongqing Medical University;2007-05
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 PAN Hai-tao.ZHENG Qi-xin.GUO Xiao-dong.et al.Department of Orthopedics.Affiliated Union Hospital.Tongji Medical College.Huazhong University of Science and Technology.Wuhan 430022.China;Transcient expression of rabbit bone marrow stromal cells transfected with green fluorescent protein gene mediated with RGD,containing peptide as gene delivery system[J];International Journal of Biomedical Engineering;2006-03
2 Wang Xiao-Jun~1,CUI Lian-qun,LI Ji,WANG Min,LIU Ji-dong(1Shandong Provincial Qianfoshan Hospital,Jinan 250014,P.R.China);Isolation and cultivation of Rat BMMSCs and transferring of EGFP[J];Shandong Medical Journal;2007-02
3 WANG Qunhua, LING Bin , ZHAO Weidong, et al (Key Laboratory of Molecular Medicine of Anhui Province, Anhui Provincial Hospital, Hefei Anhui 230001, China);Study on Establishment of Animal Model of Endometriosis[J];Journal of Practical Obstetrics and Gynecology;2005-08
4 Tan Xianjie,Liu Dongyuan,Lang Jinghe,et al.Department of Obstetrics and Gynecology,Peking Union Medical College Hospital,Beijing 100730;The exploration of the isolation and culture of eutopic endometrial glandular epithelial cell and its stromal cell as an in vitro model of endometriosis.[J];Current Advances In Obstetrics and Gynecology;2002-01
5 LIU Xi-qiang1, HUANG Hong-zhang1, ZHANG Bin1, PAN Chao-bin1, YU Dong-sheng2.(1.Department of Oral & Maxillofacial Surgery, Second Affiliated Hospital, Sun Yat-sen University. Guangzhou 510120; 2.Department of Oral & Maxillofacial Surgery, Guanghua School of Stomatology, Sun Yat-sen University. Guangzhou 510060, Guangdong Province, China);Green fluorescent protein gene transferred with polyamidoamine dendrimers into tongue carcinoma cells[J];China Journal of Oral and Maxillofacial Surgery;2005-01
6 SHI Wei, CHEN Jian, LIU Dao-kun, et al. Department of Neurosurgery, First Affiliated Hospital, Nantong Medical College, Nantong 226001, China;Changes of neural behavior and hippocampal neurons following traumatic brain injury in rats[J];Chinese Journal of Traumatology;2003-04
7 WANG Dan-bo, ZHANG Shu-lan, NIU Hui-yan, LU Jing-ming . Department of Obstetrics and Gynecology, Second Affiliated Hospital of China Medical University, Shenyang 110004, China;Study of tissue morphology and vascular endo thelial growth factor and matrix metalloproteinase-9 gene expressions in nude mouse endometriosis model[J];Chinese Journal of Obstetrics and Gynecology;2004-11
8 XIE Na, WANG Xiao-yan, ZHANG Qiong, LIN Yuan-yuan, LIN Ju-sheng. Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Construction and expression of EGFP controlled HBV promoters in eukaryotic cells[J];Chinese Journal of Hepatology;2006-04
9 ZHOU Zheng, YIN Jin-bo, YANG Hui, et al. Department of Newosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China;Intracerebral transplantation of TH -gene modified neural stem cells for Parkinson's disease[J];Chinese Journal of Neurosurgery;2003-05
10 TENG Yong*,HU Yun-yu,WANG Zhen,et al.Department of Orthopadics,Xijin Hospital,Fourth Military Medical University,Xi'an 710032,China;Differentiation of human bone marrow-derived mesenchymal stem cells into chondrocytes in vitro[J];Chinese Journal of Experimental Surgery;2005-02
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 ZHONG Lin,ZHONG Xiao-bo,ZHANG Yun-yan,et al.(Department of Endodontics,Affiliated Hospital of Stomatology,Chongqing Medical University,Chongqing 400015,China);Construction of eukaryotic expression vector pEGFP-N_1-BMP_2 and its expression in human periodontal ligament fibroblast cells transfected via ultrasound-mediated microbubble destruction[J];Chongqing Medicine;2010-09
2 Liao Hong-ying, Song Jiang-ping, Gu Li-jia, Weng Yi-min, Li Yun, Zhang Jian, Cai Song-wang, Yu Chao, Chen Hui-guo, Wang Cui-ping Department of Thoracic Surgery, Clinical Research Center of Chest Tumor, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 5106300, Guangdong Province, China;Inhibitory effect of small interference RNA targeting hypoxia-inducible factor 1 alpha nanospheres on human esophageal squamous carcinoma TE-1 cell growth[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2009-38
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