Expression of stem cell factor of testes was effected by cytoxan
DAI Jiangtao,et al(Department of Prediatric Urology,Children′s Hospital,Chongqing Medical University)
Objective: To assess the effect of cytoxan results on testes by the expression of stem cell factor(SCF) and repopulation index of testes in rats.Methods: 9d male Wistar rats were injected intraperitoneally with cytoxan of large,medium,small doses.After 24h,4w,8w,SCF was detected by RT-PCR and repopulation index in 4w was counted by microscop of testes in rats.Results:(1) With increase of age,soluble SCF(sSCF)of control groups was not significantly different(P0.05),but transmembrane SCF(mSCF) of control groups were significantly increased(P0.05).(2) Expression of SCF of experimental groups and control groups were compared with at the same period,sSCF were not significantly different(P0.05),but mSCF decreased significantly(P0.05),as in each experimental groups.(3) SCF were compared with same dose at three periods in experimental groups,sSCF were not significantly different(P0.05),mSCF of large doses groups were not significantly different(P0.05),mSCF of medium and small doses group in 24h were significantly lower than that of 4w and 8w,that of 4w and that of 8w was not significantly different(P0.05).(4) Repopulation index of experimental groups in 4w were significantly lower than that of control groups(P0.05),meanwhile,it was negatively correlated with doses.Conclusions: The toxicity of cytoxan can reduce the expression of mSCF of testes in Wistar rat,which resulted in decrease of repopulation index,it maybe one of the effect that cytoxan injuried seminiferous function.