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The damage of hyperglycemia to the neurons following traumatic brain injury

WANG Liang,et al (Department of Neurosurgery,the First Affiliated Hospital,Chongqing Medical University)  
Objective:To explore the damage of hyperglycemia to the neurons.Methods:Adult male SD rats were randomly divided into 3 groups:normal control group,traumatic brain injury group and insulin treated group,the blood glucose concentrations of the rats were measured before injury and when they were killed.The method of RT-PCR was applied to detect the expression of GLUT-3 genes.The change of GLUT-3 protein was detected by western-blot.The changes of NSE and NSE positive-Staining cells were examined by immunofluorescence staining Longa,balance test and screen test score were used to assess neural fnuctional defect.Results:The blood glucose concentration only increased markedly in traumatic brain injury group.through the expression of GLUT-3 and single cell NSE increased in the injured cortical both in traumatic brain injury group and insulin treated group,there was much more expression of GLUT-3 and single cell NSE but less NSE positive-Staining cells and neural functional defect in the insulin treated group at 12,24,48,72 h after injury.Meanwhile there were no changes were found in the uninjured side of each group.Conclusion:The hyperglycemia after traumatic brain injury increased the damage to the neurons by decreasing the expression of GLUT-3.
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