Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Zoonoses》 2003-05
Add to Favorite Get Latest Update

Development of Gold immunochromatographic assay(GICA)for detecting the antibodies against Taenia Solium cysticercosis

TANG Yu-de,LU Cheng-ping (East-China Institute for Medical Biotechnology,Nanjing 210002)  
Aim To develop a simple,fast,easy-to-do and new detecting method of the Abs against Taenia Solium cysticercosis for country veterinarian.Methods Gold immunochromatographic assay(GICA)was established by combining colloidal gold technique with immunochromatographic assay.Compared with enzyme-linked immunoabsordent assay(ELISA),its sensitivity,specificity and stability were evaluated.Results The optimum marked amount of Ab of one-milliliter colloidal gold was 30.8μg,the optimum concentration of the coating antigen was 1mg/ml.The Concordance was 96.8%(P0.05)between the developed GICA and ELISA for detecting the antibodies against swine cysticerci.The results were negative for detecting the sera of normal pigs and other disease pigs.The whole detecting time was 5 to 15min.GICA strips coated with antigen and antibody can be stored at 4℃,room temperature for 105d and 37℃ for 45 d,repectively.Conclusion GICA detecting the antibodies against swine cysticerci is convenient,rapid,sensitiv,specific and stabile.
【CateGory Index】: R446.6
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 8 Hits
1 SUN Su-ling(Anhui Science and Technology University,Fengyang,Anhui 233100);Development of Colloidal Gold Rapid Diagnostic Stripe against Chicken Infectious Anaemia[J];Journal of Anhui Agricultural Sciences;2008-12
2 ZHOU Dong-ming,TANG Yu-de,YU Xing-ming,QIAO Jian-zhong(Center for Disease Control and Prevention of Nanjing Military Command,Nanjing 210002,Jiangsu,China);Development of gold immunochromatographic assay(GICA) for rapid detection of Salmonella typhimurium[J];Journal of Southeast China National Defence Medical Science;2007-03
3 TIAN Zi-yu1, CAI Ya-nan2,CAI Hong-mei1,GAO Ming1,WANG Wei-wei1, ZHAO Quan2 (1.Jilin Academy of Agricultural Sciences, Changchun Jilin 130124 ; 2.Jilin Agricultural University ,Changchun Jiling 130118);Research Progress on Immunity Diagnose and Determination Methods of Cysticercosis cellulosae Disease[J];Jilin Animal Husbandry and Veterinary Medicine;2008-01
4 WANG Yu-quan1,MA Li-bin2,YU Qiu-lian3(1.Institute of Animal Sciences of Tongliao City,Tongliao 028000,China;2.Animal Detection Station of Korchin District of Tongliao City,Tongliao 028000,China;3.Liaoning Province Kangping County Animal Health Supervision Station,Kangping 110500,China);Construction of Animal Adenovirus Vectors-A Review[J];Journal of Inner Mongolia University for Nationalities(Natural Sciences);2009-02
5 WANG Wei-fang1 LI Qing-mei2 GUO Jun-qing2 LEI Ji-lin1(1Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,266071)(2Henan Key Laboratory for Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002);Progress and prospect of colloidal gold-based immunochromatographic lateral flow assay and its application in aquaculture[J];Progress in Fishery Sciences;2010-03
6 WANG Feng-qiang~(1,2),JIANG Bei-yu~2,LIU Ming-ze~3,ZHAO Yu-jun~1,MI Feng-quan~1,LIU Jian~4(1.Shenyang Agriculture University Veterinary and husbandry sciences College,shenyang 110061,China;2.Beijing Institute of verinary andhusbandry sciences;3.Beijing Xibo Medical-Tech Corporation LTD;4.Liaoning Province Yikang Bio-Product Company);Immunogold Labeling Technique Applied in Disease Diagnosis of Veterinary[J];Journal of Animal Science and Veterinary Medicine;2005-06
7 XU Peng, ZHANG Xiao-lei, ZHAO Quan (College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China);Advanced on the study of immunodiagnostic methods for cysticercosis[J];Journal of Pathogen Biology;2006-02
8 ZHANG Shao-hua1,LUO Xue-nong1,ZHENG Ya-dong1,LI Ke-sheng2,JING Zhi-zhong1,JIANG Tao1,ZHAO Song-bo1,CAI Xue-peng1(1.Key Laboratory of Veterinary Parasitology of Gansu Province,Key Laboratory of Veterinary Public Health of Ministry of Agriculture,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046;.Biotechnique Center,Medical Academy of Gansu,Lanzhou 730050,China);Development of gold immunochromatographic strip using the recombinant protein TSOL18 for rapid diagnosis of swine cysticercosis[J];Chinese Journal of Zoonoses;2009-10
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 CUI Zhuo,WANG Shu min (Department of Infectious Diseases,Affiliated Hospital of Bengbu Medical College,Anhui 233004,China);Real-time fluorescence quantitative PCR in determination of hepatitis B virus DNA[J];Journal of Bengbu Medical College;2004-01
2 Hao Jianhun,Xu Xia,Wu Junying (Department of Clinical Microbiology and Immunology, Bengbu Medical College, Anhui)Tian Weiquan,Li Zhijun (Department of Infectious Diseases,Affiliated Hospital of Bengbu Medical College, Anhui);DETERMINATION OF CIRCULATING ANTIGEN IN CEREBRAL CYSTCERCOSIS PATIENTS USING McAb-BASED ELISA[J];ACTA ACADEMIAE MEDICINAE BENGBU;1994-01
3 He Kongwang Wu Jitang Lin Jihuang Jiang Jieyuan( Institute of Animal Husbandry and Veterinary Medicine Jiangsu Academy of Agricultural Sciences, Nanjing);REVERSE INDIRECT HEMAGGLUTINATION TEST FOR DETECTING ROTAVIRUS ANTIGEN IN FECAL SAMPLES AND ANTIBODY IN SERUM[J];Chinese Journal of Virology;1989-01
4 Zhou Weisong Liu Xiufan( Department of Animal Science and Veterinary Medicine, Jiangsu Agricultural College );NEUTRALIZATION NEWCASTLE DISEASE VIRUS BY MONOCLONAL ANTIBODIES[J];Chinese Journal of Virology;1991-01
5 Sun Yi Chen Boquan ( Institute of Virology, Chinete Academy of Preventive Medicine, Beijing );STUDY ON THE "EARLY DEATH" SYNDROME IN MICE BY USING MONOCLONAL ANTIBODIES TO RABIES VIRUS[J];Chinese Journal of Virology;1991-02
6 LIU Hong-qi,HUANG Yong,CHENG Jian,PENG Da-xin,JIA Li-jun, ZHANG Ru-kuan,LIU Xiu-fan (Key Laboratory of Animal Infectious Diseases of Ministry of Agriculture,Yangzhou University,Yangzhou 22500,China);Genetic Mutations of the Hemagglutinin Gene of H_9N_2 Subtype Avian Influenza Viruses under the Selective Pressure of Vaccination[J];Chinese Journal of Virology;2002-02
7 YAN Yu-he~ 1, SHAO Yi-ming~ 1, PAN Pin-liang~ 1, JIA Bin~ 2, SHEN Rong-xian~ 2(1. NARL, National Center for AIDS Prevention and Control, Beijing 100050, China; 2. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China);Studies on Structure and Function of dUTPase of EIAV-Structural Analysis, Expression of Genes Encoding dUTPase from Virulent and Vaccine EIAV Strains and Characterization of Recombinant Proteins[J];Chinese Journal of Virology;2004-02
8 LIU Hong-wei, ZHANG Bin, GE Hong,et al. (Academy of Military Medical Sciences,Beijing 100071,China);Whole Blood Rapid Immunochromatographic Assay of Serum Myoglobin[J];Labeled Immunoassays and Clinical Medicine;2001-04
9 WANG Yu-jin, WANG Yu-zhu, YANG Shu-hao, et al. (Zhengzhou, Biocell Biotechnology Institute, Zhengzhou 450001, China);Colloidal Gold Immunochromatographic Assay for Carcinoembryonic Antigen in Serum[J];Labeled Immunoassays and Clinical Medicine;2003-03
10 Chu Xuntao1,Lu Shengkai2,Dong Yongqin3et al. (1 Laboratory of Medical Technology,Shanghai Second Medical University,Shanghai 200023; 2 Shanghai Centre for Clinical Laboratory Sciences,Shanghai 200052; 3Department of Medical Laboratory Sciences,Shanghai;Study on Immunogold Quantitative Assay for Microalbuminuria[J];LABELED IMMUNOASSAYS AND CLINICAL MEDICINE;1999-02
【Secondary References】
Chinese Journal Full-text Database 9 Hits
1 FENG Xiang-hui1,WANG Jian-yong2,BAO Yong-zhan1,ZHAO Yue-lan2,ZUO Yu-zhu1,QIN Jian-hua1(1. College of Animal Science and Technology,Hebei Agriculture University,Baoding,Hebei,071001,China;2. College of Chinese Traditional Veterinary Science,Hebei Agriculture University,Dingzhou,Hebei,073000,China);Establishment and Preliminary Application of Immunochromatographic Strips for Rapid Detection of Antibodies Against M.bovis[J];Progress in Veterinary Medicine;2010-02
2 HU Ren-jian,CAI Jia-li,XU Jia-yuan,ZHANTG Gui,LIANG Xiao-yuan,LI Xia-qing,GUO Ping,ZHENG Yang(School of Chemical and Biological Engineering,Chongqing University of Technology,Chongqing 400050,China);Preparation of HPV16 Immane Colloidal Gold Diagnostic Strip[J];Journal of Chongqing University of Technology(Natural Science);2010-02
3 WANG Qing,HU Jian-he,ZHENG Su-ling,WU Yu-ping (Henan Institute of Science and Technology,Xinxiang,Henan,453003,China);Advance in Live-Vector Vaccine of CSFV E2 Gene[J];Progress in Veterinary Medicine;2010-07
4 GUO Quan-hai1,2,MENG Jun3,WU De-feng2(1.Shangqiu Vocational and Technical College,Shangqiu 476000,China;2.Fujian Agricultural and Forestry University,Fuzhou 350002,China;3.Zhengzhou Light Industry College,Zhengzhou 450002,China);Research Advance in Chicken Infectious Anaemia Disease[J];Acta Agriculturae Jiangxi;2009-10
5 CHEN Jian-hong1, GUO Jun-qing2 (1.Health and Quarantine Station of Zhenze of Wujiang City of Jiangsu Province, Wujiang 215231, China; 2. Nanjing Agricultural University, Nanjing 210095, China);Raising Administration of SPF Chicken and Application of Isolator[J];Animal Husbandry and Feed Science;2010-01
6 ZHENG Xiao-yan,GU Jun-chao.(Beijing Institute of Tropical Medicine,Beijing Friendship Hospital Affiliated to Capital Medical University,Beijing 100050,P. R. China);Advance in the research of diagnosis and treatment of cysticercosis[J];China Tropical Medicine;2009-11
7 GE Xiao-qin, LI Hong-jun, WANG Wen-ju, et al(Institute of Medical Biology, Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China);Construction and Expression of Bicistronic Recombinant Adenovirus with P1 and 3CD Genes of Enterovirus 71[J];Chinese Journal of Biologicals;2010-04
8 HUANG Jiang-shan*,ZHANG Meng-zhang,CHEN Zhong-de,et al.(*The First Hospital of Xiamen,Fujian Medical University,Xiamen,Fujian 361003,China);Study of Colloidal Gold Strip in Detecting the Antibody of Rabies[J];Chinese Journal of Natural Medicine;2009-04
9 XU An-jian,GU Jun-chao(Beijing Tropical Disease Institute,Beijing Friendship Hospital,Beijing 100050,China);Progress of molecular biology research on cysticercosis[J];Journal of Pathogen Biology;2010-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved