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《Chinese Journal of Zoonoses》 2008-10
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Evaluation of the effectiveness of anti-retroviral therapy on AIDS patients in Fujian province and the current status of their drug-resistance pattern

YAN Ping-ping,XIE Mei-rong,WU Shou-li,XIA Ping-chan,ZHENG Jian,YAN Yan-sheng(Fujian Center for Disease Control and Prevention,Fuzhou 350001,China)  
To evaluate the effectiveness of anti-retroviral therapy on AIDS patients in Fujian province and the current status of their drug-resistance pattern in order to provide experimental evidences for anti-reteroviral therapy,counts of the CD4 + T lymphocytes in the peripheral blood of 25 AIDS patients receiving anti-retroviral therapy and the virus load of HIV in plasma of these patients were determined,in which the whole sequence of gene coding the pol protein of HIV and the partial reverse transcriptase gene were amplified by RT-PCR,and then the amplified fragments were edited by using Contig Express software and submitted to the website http//hivdb.Stanford.edu./to analyze the drug-resistance status.Meanwhile,their phylogenesis was analyzed by Bio-Edi,clustal and Mega softwares.It was found that 68%(17/25)of the patients treated with anti-retroviral therapy showed the level of virus load(VL)below 1000 cp/ml and in 40%(10/25)of the treated patients the VL even dropped below the detectable limit(50 cp/ml).The amplified fragments were obtained in 10 cases of patients treated by means of RT-PCR and their sequence subtype analysis demonstrated that 7 cases belonged to the HIV-1 AE,2 cases were HIV-1 B and one was HIV-1 D.As demonstrated by the results in drug-resistance analysis,the sequences obtained from 10 cases was found to be sensitive to the action of protease inhibitors(PI),however,those from 8 cases were highly resistant to at least 3 anti-retroviral drugs of nucleotide reverse transcriptase inhibitors(NRTI)and non-nucleotide reverse transcriptase inhibitors(NNRTI).The commonly observed loci of mutations were G190A,K103N and M184V,thus causing high or medium level of resistance to NVP and 3TC.From these observations,it is evident that the types and amount of developing mutations on drug-resistance in AIDS patients during treatment with anti-retroviral therapy are definitely increased,although their immune function would be gradually recovered after treatment.The result of these will be the development of drug-resistance to certain first-line drugs.Therefore,it is necessary to motivate patients to keep them highly compliance to the first line regimes and to have the second line regimes available besides performing timely evaluation of the drug-resistance of patients.
【Key Words】: AIDS antiretroviral therapy drug resistance
【CateGory Index】: R512.91;R446.5
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【References】
Chinese Journal Full-text Database 1 Hits
1 Wang Yide,Wang Xiangyang,Li Wei,et al Linquan County Center for Disease Control and Prevention,Linquan 236400 School of Public Health,Anhui Medical University,Hefei 230032;Evaluation of second-line antiretroviral therapy effect on AIDS patients in Linquan County[J];Anhui Medical Journal;2009-09
【Citations】
Chinese Journal Full-text Database 2 Hits
1 LI Tai sheng,et al;Immune reconstitution in AIDS patients during highly active antiretroviral therapires[J];Basic Medical Sciences and Clinics;2001-01
2 YAN Ping-ping, XIE Mei-rong, ZHENG Jian, PAN Yun-jiao, YAN Yan-sheng(Fujian Provincial Center for Disease Prevention and Control, Fuzhou 350001,China);The clinical progression of individuals with HIV infection in Fujian and their effectiveness with anti-retroviral therapy[J];Chinese Journal of Zoonoses;2007-05
【Co-citations】
Chinese Journal Full-text Database 3 Hits
1 YANG Hong-ying1,SUN Jing2. 1.Hubei Global Fund AIDS Program Office,Wuhan 430079,China;2.School of Public Health,Wuhan University,Wuhan 430071,China;Evaluation of status of anti-retroviral treatment for HIV/AIDS patients in four Global Fund AIDS Program counties in Hubei Province[J];Chinese Journal of Disease Control & Prevention;2009-04
2 WANG Jie WU Xin-fang Guangan’ men Hospital,China Academy of Chinese Medical Sciences;Immune Reconstitution for AIDS Patients and TCM Advantages in Intervening It[J];Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai;2009-04
3 YAN Ping-ping, XIE Mei-rong, ZHENG Jian, PAN Yun-jiao, YAN Yan-sheng(Fujian Provincial Center for Disease Prevention and Control, Fuzhou 350001,China);The clinical progression of individuals with HIV infection in Fujian and their effectiveness with anti-retroviral therapy[J];Chinese Journal of Zoonoses;2007-05
【Co-references】
Chinese Journal Full-text Database 1 Hits
1 XIAO Mingying,BI Zhigang,MA Jie,JIANG Shenrong(Baoshan Teaching Hospital of Dali University,Baoshan,Yunnan 678000,China);A Clinical Study of Free Anti-virus Treatment of AIDS[J];Journal of Dali University;2008-12
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 LI Tai sheng,et al;Immune reconstitution in AIDS patients during highly active antiretroviral therapires[J];Basic Medical Sciences and Clinics;2001-01
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