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Detection and analysis of diarrheagenic Escherichia coli from stool specimens of patients with infectious diarrhea in Changzhi area

PANG Hui,ZHAO Ai-lan,BAI Xiang-ning,WANG Li-bing,GUO Bu-ping,XIONG Yan-wen(Changzhi Medical College,Shanxi 046000,China)  
The diarrheagenic Escherichia coli from diarrhea patients in Changzhi area,Shanxi Province were detected and characterized in this study.Feces from diarrhea patients were enriched in EC broth.Diarrheagenic Escherichia coli were primary screened by multiple polymerase chain reaction(PCR) using 7 virulence genes of eaeA,aggR,ipaH,stx1,stx2,lt,stIb and the gene of 16S rRNA rrs.Then,the diarrheagenic Escherichia coli were isolated from positive enrichment medium.Isolates were conducted with biochemical identification,virulence genes detection and sero-groups testing.A total of 170 fecal specimens were collected and 20 diarrheagenic Escherichia coli were isolated,among which 11 strains were enteropathogenic Escherichia coli(EPEC) with all atypical EPEC,7 strains were enteroaggregative Escherichia coli(EAEC),and 2 strains were enterotoxigenic Escherichia coli(ETEC).Results showed that the infection of diarrheagenic Escherichia coli was mainly in children aged less than 5 years in Changzhi area.In addition to EPEC,EAEC occupied a large proportion.It's suggested that the test of virulence genes should combine with traditional sero-typing in the screening of diarrheagenic Escherichia coli infection.
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