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《Journal of China-Japan Friendship Hospital》 2012-03
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Protective effect of FK506 on the lung mitochondrial damage following traumatic stress of amputation in rats

ZHANG Hai-feng,ZHANG Ying,REN Qing-ai First Department of Comprehensive Surgery,South Building,General Hospital of PLA,Beijing 100853,China  
Objective:To investigate the lung mitochondrial damage of rats induced by lower limb amputation and the protective effect of tacrolimus(FK506).Methods:Twenty-four male SD rats were randomly divided into 3 groups(8 each):control group,6 hours after amputation group(traumatic group)and intervention with FK506 after amputation group(intervention group).The model of left lower limb amputation was established in the rats of traumatic group and intervention group.FK506 was given immediately by intraperitoneal injection in intervention group.The rats were sacrificed 6 hours after trauma,and the mitochondria were extracted from lung.The mitochondrial oxygen consumption of stage 3(ST3)and stage 4(ST4),respiratory control ratio(RCR),membrane potentials and total ATPase activity were detected.The morphological changes in lung mitochondria were observed by electron microscopy.Results:Compared with control group,the mitochondrial oxygen consumption of ST3,RCR,membrane potentials and total ATPase activity decreased(P0.05).Compared with traumatic group,the mitochondrial oxygen-consumption of ST3,RCR,membrane potentials and total ATPase activity increased(P0.05).Ultrastructural examination revealed swelling and disorganization of cristae in mitochondria in traumatic group and only slight swelling of mitochondria was found in FK506 group.Conclusion:The stress of amputation trauma may result in damage of lung mitochondria,and FK506 seems to be able to attenuate the damage.
【CateGory Index】: R965.1
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