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《Journal of Fishery Sciences of China》 2007-06
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Effects of high temperature stress on chlorophyll fluorescence kinetics of Dunaliella salina and Pyramimonas sp.

LIANG Ying,FENG Li-xia,TIAN Chuan-yuan,WANG Shuai(The Key Laboratory of Mariculture of Ministry of Education,Ocean University of China,Qingdao 266003,China)  
Chlorophyll fluorescence can be readily measured by a range of fluorometers,which provides a quick,non-invasive,non-destructive assessment of the efficiency of photochemical conversion by PSⅡ,thus it has become an increasingly powerful tool and widely used techniques in studies of photosynthesis in marine algae,particularly for investigations of stress physiology of microalgae.There are several chlorophyll fluorescence parameters that have been correlated to photosynthetic processes.One of the most commonly used parameters is the ratio of variable to maximal chlorophyll fluorescence(Fv/Fm),the maximal photochemical efficiency of PSⅡ or the maximal quantum yield of PSⅡ,which has been shown to be a sensitive indicator of photosynthetic performance and photoinhibition.Moreover,other fluorescence parameters,such as actual photochemical efficiency of PSⅡ in the light(ΦPSⅡ=yield),the relative electron transport rate(rETR),the photochemical quenching(qP) and the non-photochemical quenching(NPQ) are also used in investigating photosynthesis by many plant physiologists.Microalgae plays an important role in aquatic primary productivity.Some microalgal species,which have high nutritional value and can grow well in large-scale cultures,are commonly used in many mariculture systems as food for certain aquatic animals.The growth and photosynthesis of microalgae are dependent on light,salinity,temperature and nutrient.Among the different culture conditions,temperature is one of the most important environmental factors limiting algae production.In this paper,the modulated chlorophyll fluorescence kinetics of Dunaliella salina and Pyramimonas sp.under high temperature stress(35-50 ℃) were investigated with Water-PAM chlorophyll fluorometer.The results showed that the parameters Fv/Fm,Fv/Fo,ΦPSⅡ,ETR and qP decreased under high temperature stress in the two microalgae.The changing degree of the above parameters increased with the increase of temperature and exposure time.NPQ in the two microalgae exhibited different pattern of variation under high temperature stress.NPQ value in D.salina first increased then decreased,while that in Pyramimonas sp.first decreased then increased at 40 ℃ and only decreased at 35 ℃ and 45 ℃ during stress.The decrease of Fv/Fm and Fv/Fo indicated that high temperature stress resulted in major impairment of photochemical efficiency as well as inhibition of potential activity of PSⅡ.ΦPSⅡ represents the actual photochemical efficiency of PSⅡ reaction centers of lightadapted cells.The decline in ΦPSⅡ indicated that high temperature affected PSⅡ electron transport and equivalented(NADPH and ATP) generation,leading to inhibition of carbon fixation and assimilation by the primary photochemical reactions.High temperature stress also resulted in decrease in ETR,which indicated inhibition and/or downregulation of photosynthesis.qP showing the proportion of light excitation energy converted to photochemical act by the active PSⅡ reaction centers,representing the proportion of open PSⅡ reaction centers.The decreased qP in high temperature-stressed cells might indicate a reduced photochemical utilization of the absorbed light energy and an increased proportion of closed/inactive reaction centers,which was in accordance with the decrease in ΦPSⅡ.Non-photochemical quenching(NPQ) is used to describe all quenching processes of PSⅡ chlorophyll fluorescence that are not related to photochemistry.The first increases in NPQ might indicate increase of thermal dissipation of electron transport,thus protecting microalgal cells against damage from excess radiant energy absorption.The subsequent decline of NPQ might be related to the serious damage of physiological function vesulting in lose of the ability to dissipate excess excitation energy by energy-dependent quenching mechanisms.Several previous studies have reported that chlorophyll fluorescence could be potentially used as a tool for temperature tolerance screening in some microalgal species.However,only limited microalgal species have been studied and a lot of further works with different microalgal species need to be done to confirm whether chlorophyll fluorescence could be used as a tool for temperature tolerance screening in microalgae.
【Fund】: 中国海洋大学海水养殖教育部重点实验室开放课题资助(200603)
【CateGory Index】: Q945
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