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《Journal of Fishery Sciences of China》 2008-04
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Immunoregulation of chitosan on Ctenopharyngodon idella by the pathway of thyroid hormone

HUA Xue-ming,YAN Da-wei,ZHOU Hong-qi (Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education, Shanghai 200090,China)  
It is well known that chitosan can enhance aquatic animal’s immunity,while relatively few researches were on immunoregulation mechanism. With the development of immunology,the relationship between endocrine system and immune system was gradually known,but it’s doubt there is influence of thyroid hormone on immunity capacity and that chitosan can regulate immunity capacity by the pathway of endocrine system in Ctenopharyngodon idella. In the present study,in order to understand the influence of thyroid hormone on immunity of grass carp Ctenopharyngodon idella and possible immunoregulation mechanism of chitosan by the pathway of thyroid hormone,diets containing thyroid hormone[0,1,5,10 mg (thyroid hormone)/kg (feed)] or chitosan ( 0%,0.25%,0.50%,0.75% and 1.00%) respectively were formulated to feed grass carp [initial body weight (1.04±0.01)g] for 70 days in indoor recirculation aquarium. The amount of thyroid hormone in serum,the nonspecific immune function such as lysozyme activity and NO content of anterior kidney and spleen, phagocytic activity of peripheral leucocyte were determined. The results indicated that there was significant effect of dietary thyroid hormone on the amount of T3 and T4 in serum,the nonspecific immune function( lysozyme activity of anterior kidney and spleen and NO content of anterior kidney) (P0.05). T3 (1.31 μg/mL) and T4 (3.14 μg/mL)content in serum,lysozyme activity of anterior kidney and spleen (77.06 μg/g,55.88 μg/g),the NO content (9.36 μmol/g prot)of anterior kidney of 5 mg thyroid hormone kg-1 feed treatment were significantly higher than those of the control group (P0.05),and similar change trend betweenT3,T4 content in serum and nonspecific immunity implied that thyroid hormone could modulate immunity of the tested fish. The results also showed that there was significant effect of chitosan on the amount of T3 and T4 in serum,the nonspecific immune function( lysozyme activity of anterior kidney and spleen and NO content of anterior kidney,phagocytic activity of peripheral leucocyte) (P0.05) .The amount of thyroid hormone in serum and the nonspecific immune function firstly increased and then decreased with the increased dietary chitosan;the amount of T3,T4 (1.43 μg/mL, 5.22 μg/mL) in serum,lysozyme activity of anterior kidney and spleen (77.06 μg/g,57.65 μg/g),the NO content (9.52 μmol/g prot) of anterior kidney,phagocytic activity of peripheral leucocyte (46.00%) of 0.50% chitosan treatment were significantly higher than those of the control group (P0.05). Summing up all the results,we can draw the conclusion that chitosan can regulate immunity of the tested fish by thyroid hormone. Since the significantly enhanced immunity was found in the 0.50% chitosan group,appropriate dosage of chitosan was 0.50% of the diets in this experiment.
【Fund】: 上海市重点学科建设项目(Y1101);; 上海高校选拔培养优秀青年教师科研专项基金(677170701);; 上海海洋大学博士启动基金(05-25)
【CateGory Index】: S963
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