The Purification and Restriction Map of Liver Mitochondrial DNA from Elaphe radiata and Dendrophis boiga boiga
Luo Jinxian;Chen Shouchai;Wu Yingji Department of Biology
Liver mitochondrial DNA from Elaphe radiata and Dendrophis boiga boigawere purified by gel filtration on a sepharose 4 B column or by ultracentrifu-gation in 1M NaCl. Their molecular sizes are 17.75Kb and 19.70Kb respectivelyas estimated by agarose gel electrophoresis. The mtDNAs were digested with re-striction endonucleases EcoRI, XbaI, BamHI and BglII and, 1, 1, 2,and 3 cutt-ing sites were found on Elaphe radiata mtDNA and 4, 1, 1 and 2 sites on De-ndrophis boiga boiga mtDNA. The restriction maps were built according to thedata from single, double and partial digestions of these mtDNAs by the above 4restriction endonucleases.