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《Acta Scientiarum Naturalium Universitatis Sunyatseni》 2001-06
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Study of Activation and Spectrum of Catalase from Aspergillus niger

LIU Jian-zhong,LIU Jie,WENG Li-ping,JI Liang-nian (The Key Laboratory of Gene Engineering of Minister of Education, Zhongshan University, Guangzhou 510275, China)  
The activation of catalase from Aspergillus niger by thioglycolic acid was investigated. Thioglycolic acid showed positive effect on catalase activity and the optimal concentration of thioglycolic acid was 0.6 mmol/L. The reasons of the activation of thioglycolic acid were also investigated by the determination of kinetic constants, the absorbance and emission fluorescence. The kinetic parameters, indicated that the addition of thioglycolic acid enhanced the affinity and specificity of catalase. The heme environment (active site) and tryptophane resides of catalase were altered by thioglycolic acid.
【Fund】: 广东省自然科学基金资助项目 (970 840 0 0 12 78);; 高等学校重点实验室访问学者基金资助项目
【CateGory Index】: Q554.6
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