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《Acta Scientiarum Naturalium Universitatis Sunyatseni》 2001-06
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Study of Activation and Spectrum of Catalase from Aspergillus niger

LIU Jian-zhong,LIU Jie,WENG Li-ping,JI Liang-nian (The Key Laboratory of Gene Engineering of Minister of Education, Zhongshan University, Guangzhou 510275, China)  
The activation of catalase from Aspergillus niger by thioglycolic acid was investigated. Thioglycolic acid showed positive effect on catalase activity and the optimal concentration of thioglycolic acid was 0.6 mmol/L. The reasons of the activation of thioglycolic acid were also investigated by the determination of kinetic constants, the absorbance and emission fluorescence. The kinetic parameters, indicated that the addition of thioglycolic acid enhanced the affinity and specificity of catalase. The heme environment (active site) and tryptophane resides of catalase were altered by thioglycolic acid.
【Fund】: 广东省自然科学基金资助项目 (970 840 0 0 12 78);; 高等学校重点实验室访问学者基金资助项目
【CateGory Index】: Q554.6
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【References】
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1 ZHANG Zeng-xiang1,2,WANG Wei1,HAO Jian-hua1,NIU Rui1,SUN Mi1(1.Laboratory of Marine Products and Enzyme Engineering,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;2.College of Food Science & Technology,Shanghai Ocean University,Shanghai 201306,China);Isolation and identification of catalase-producing strain CE-1[J];Marine Sciences;2010-11
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1 Liu JianzhongBiotechnology Research Center,Zhongshan University,Guangzhou 510275,China Yang Huiying Zhao Jilun Weng Liping Ji Liangnian;A Study on Axial Ligands of Model Enzyme to Activize Catalase A New Method of Enhancement Natural Enzyme Activity[J];ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS SUNYATSENI;1998-02
【Co-citations】
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1 WANG Dan1,2,ZHANG Qiang2,LI Wang2,QI Nanchang3,GUO Chunxiao1,YANG Zhirong1,ZHANG Jie1,* 1Life Science College,Sichuan University,Chengdu 610065,Sichuan,China 2Department of Biomedicine,Chengdu Medical College,Chengdu 610083,Sichuan,China 3Department of Medical Examination,Chengdu Medical College,Chengdu 610083,Sichuan,China;Asymmetric Reduction of Ethyl Pyruvate Catalyzed by Yeast Resting Cells to (S)-Ethyl Lactate[J];Chinese Journal of Catalysis;2011-06
2 XIE, Xiu-Yin WANG, Zhi-Yong LIU, Yu-Wen WANG, Cun-Xin  (College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072);Thermokinetic Studies on the Cooperative Inhibition of Catalase by L-Ascorbic Acid and Cu~(2+)[J];Acta Chimica Sinica;2003-03
3 Peng Xiao-Bin (Department of Applied Chemistry, South China University of Technology,Guangzhou 510641) Liang Shi-Qiang (Zhongkai College of Agricultural Technology, Guangzhou 510225);CD Spectrum of Chiral Threonine Modified Porphyrinatozinc (II)[J];Acta Physico-chimica Sinica;2001-03
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1 Jin Zhiyu,Ma Songke,Yang Rudao Yunnan Provincial Health & Anti-epidemic Center,Kunming 650022;THE GENOTOXICITY STUDY OF CHCl 3 AND CCl 4 USINGMICRONUCLEUS TEST IN VICIA FABA ROOT TIP CELLS[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1997-03
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