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《Chinese Journal of Experimental Traditional Medical Formulae》 2006-08
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Determination of Rutin and Quercetin in Flos Sophorae with Thin Layer Chromatogram Scanner

ZHOU Xuan,SONG Fen-yun,ZHONG Zhao-jian(Department of Pharmaceutical Analysis,Guangdong College of Pharmacy,Guangzhou 510006,China)  
Objective:To develop a TLCS method for determination of rutin and quercetin in Flos sophorae.Method:A TLCS method was used.The developer was a micro-emulsion consisted of soldium dodecyl sulphate/n-C_4H_9OH/n-C_6H_(14)/H_2O/formicacid(0.27∶0.63∶0.10∶3∶0.2) and the thin layer was polyamide film.The spots of rutin and quercetin were well separated and scanned at 370nm with reference wavelength of 550nm.Results:The linear ranges of calibration curves were 0.2~3.0μg for rutin,0.1~1.0μg for quercetin.The recoveries of rutin and quercetin were 98.8% and 97.1% respectively.Conclusion:A TLCS method has been developed for the simultaneous determination of rutin and quercetin in Flos sophorae.
【CateGory Index】: R284.1
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