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《Chinese Journal of Experimental Traditional Medical Formulae》 2008-11
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Application of Polyamide Resin in Purification of Hyperoside from Hypericum perforatum L.

KE Zhong-cheng~(1),GUI Shuang-ying~2,ZHOU Ya-qiu~2(1.Huangshan University,Huangshan 245041,China;?2.Anhui College of TCM,Hefei 230031,China)  
Objective:To study the technological parameters of the purification process of hyperoside from Hypericum perforatum extrative.Methods: HPLC was used to analyze the content of hyperoside in the samples from the different technological process,and the adsorption and elution parameters were investigated.Results: The optimal parameters were as follows:the sample extract solution,whose content of hyperoside was 1.2 mg·mL~(-1),was passed through the packed column with a flow rate of 3.6 BV·h~(-1).The adsorption rate of hyperoside was 2.33 mg·g~(1),and the purity of hyperoside in the eluate reached 28.96%.Conclusion: Polyamide resin can be used to purify hyperoside in the extraction of Hypericum perforatum L.
【CateGory Index】: R284.1
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