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《Chinese Journal of Experimental Traditional Medical Formulae》 2010-16
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Determination of Chlorogenic Acid,Caffeic Acid and Mangiferin in Shiwei Dispensing Granule by HPLC

WU Huai-en1,2,LAO Shen1,WANG Wen-hui1,WEI Tao1,HUANG Hai-yan1,LIN Li-mei1(1.College of Pharmacy,Guangxi Traditional Chinese Medical University,Nanning 530001,China;2.College of Pharmacy,Chengdu Traditional Chinese Medical University,Chengdu 611137,China)  
Objective:To establish an HPLC method for the simultaneously determination of chlorogenic acid,caffeic acid and mangiferin in Shiwei Dispensing Granule.Method: Chlorogenic acid,caffeic acid and mangiferin were separated on Shim-pack VP-ODS(4.6 mm×250 mm,5 μm) column and detected at 323 nm(0~30 min) and 258 nm(30.1~45 min),with acetonitrile-0.1% phosphoric acid solution(9∶91) as mobile phase.The flow rate was 1.0 mL.min-1.Result:Chlorogenic acid,caffeic acid and mangiferin were linear within the range of 129.6~302.4 mg.L-1(r = 0.999 8),13.8~32.2 mg.L-1(r=0.999 9) and 151.2~352.8 mg·L-1(r=0.999 9) respectively.The average recovery were 98.15%(RSD 1.69%),99.93%(RSD 2.98%) and 101.43%(RSD 2.00%).Conclusion: The method is simple,reproducible,and it can be used for the quality control of Shiwei Dispensing Granule.
【CateGory Index】: R284.1
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