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《Chinese Journal of Experimental Traditional Medical Formulae》 2011-03
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Corelation between Expression of AQP1,AQP5 mRNA and Lung Injury in Rats with Lung-defensive Sluggishness Syndrome and Effect of Rhubarb on It

DENG Shi-gui,WU Li-ping,HUANG Hai-ding(Animal Laboratory Center,The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangdong Provincial Academy of Chinese Medical Sciences,Guangzhou 510006,China)  
Objective:To observe the correlation between the expression of AQP1,AQP5 mRNA and the lung injury in the rats with lung sluggishness syndrome and the regulative effect of rhubarb on it,to explore the material foundation of the lung defensive sluggishness syndrom and the mechanism of rhubarb in the prevention and treatmentof the lung injury.Method:Lipopolysaccharide(LPS)was injected into SD rats to perform lung-defensivesluggishness syndrome.Sixty rats was randomly divided into 5 groups:control group,lung-defensive sluggishnessgroup,natural recovery after 5 days group,rhubarb prevention group(9 g·kg-1·d-1 for 2 days before modelestablishment)and rhubarb treatment group(9 g·kg-1·d-1for 5 days after model establishment).Physiological andpathological staining was performed and real-time PCR was used to observe the expression of AQP1,AQP5 mRNA inthe pulmonary tissue.Result:The pulmonary edema could be showed obviously in the lung-defensive sluggishnessmodel and rhubarb could alleviate it.Compared with the control group,expression of AQP1,AQP5 mRNA of themodel group was decreased,thus the natural recovery after 5 days group was elevated;no significantly difference canbe seen between rhubarb prevention group and rhubarb treatment group.Conclusion:The pulmonary edema andabnormal expression of AQP1,AQP5 mRNA can be showed in lung-defensive sluggishness model and the rhubarb caninhibit them,which is one of the important mechanisms in protecting the lung injury of lung-defensive sluggishnesssyndrome.
【Fund】: 广东省科技厅项目(83028 2009B060300025);; 广东省中医药管理局项目(2008318 2009189);; 广州中医药大学项目(08CX82)
【CateGory Index】: R285.5
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