Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Experimental Traditional Medical Formulae》 2011-16
Add to Favorite Get Latest Update

Effect of Rhei Radix et Rhigoma on AQP_(1,5) mRNA Expression of Alveolar Type Ⅱ Cell Injured by LPS

WU Li-ping1,DENG Shi-gui2,HUANG Hai-ding1(1.Department of pharmacy,the Peoples Hospital of Ganzhou,Ganzhou 341000,China; 2.The Animal Laboratory Center of the Second Affiliated Hospital,Guangzhou University of Chinese Medicine,Guangzhou 510006,China)  
Objective: To observe effect of Rhei Radix et Rhigoma on AQP1,5 mRNA expression of alveolar type Ⅱcell(ATⅡ) injured by LPS.Method: Primary cultured alveolar type Ⅱcells were divided into 5 groups: control group: 2mL DMEM cultured medium was added;LPS injured cell model group(acute lung injury cell model group): 10 μg·mL-1 LPS was added to injure AT Ⅱ cell;serum containing Rhei Radix et Rhigoma intervention group was divided into 3 groups: each group was added the total volume of 5%,10%,20% serum containing Rhei Radix et Rhigoma.Which were collected After 4 hours of culture the mRNA expression of AQP1,AQP5 was detected by Real-Time PCR.Result: Compared with the control group,AQP1 mRNA expression of LPS injured cell model group was significantly lower(P0.05).Compared with the LPS group,20% of total volume of serum containing Rhei Radix et Rhigoma intervention group could increase AQP1 mRNA expression significantly,but not return to the normal level.Compared with the control group,AQP5 mRNA expression of LPS injured cell model group was increased.Rhei Radix et Rhigoma intervention groups could decrease AQP5 mRNA expression,but not significantly.Conclusion: The protective mechanism of Rhei Radix et Rhigoma on acute lung injury cell model by LPS may be associated with the increased AQP1 mRNA expression and the decreased AQP5 mRNA expression.
【Fund】: 广东省科技厅(83028 2009B060300025);; 广东省中医药管理局(2008318)广州中医药大学(08CX82);; 赣州市医办字[2010]53号-5)
【CateGory Index】: R285.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved