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《Chinese Journal of Experimental Traditional Medical Formulae》 2012-21
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Analysis on Correlation of HPLC Specific Chromatogram of Rhei Radix Et Rhizoma and its Ethanol Extract

ZHANG Zi-long 1,WEI Gang1,LIU Dong-hui1,HUANG Yue-chun2,YANG Li-e1,DUAN Yuan-yuan1(1.Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China; 2.The First Affiliated Hospital of Guangzhou University of TCM,Guangzhou 510405,China)  
Objective:To establish the method of specific chromatogram analysis of Rhei Radix Et Rhizoma by HPLC,and to study the correlation between Rhei Radix Et Rhizoma and its ethanol extract and to estimate the alcohol extracting technology.Method:HPLC was used with the Zorbax SB-Aq C18 column,the acetonitrile-phosphoric acid solution(φ=0.1%)(gradient elution) was employed as a mobile phase,detection wavelength was at 430 nm and 280 nm,column temperature was set at 40 ℃,and flow rate was 1.0 mL · min-1.Result:Fourteen common peaks were separated in the decoction pieces and its ethanol extract of Rhei Radix Et Rhizoma and 5 peaks were identified in 430 nm,the similarities of 8 batches of the decoction pieces and ethanol extract were over 0.96 and 0.95,the similarities of the other 2 batches were below 0.90.10 common peaks were separated in the decoction pieces and its ethanol extract and 2 peaks were identified in 280 nm,the similarities of 8 batches of the decoction pieces and ethanol extract were over 0.94 and 0.92,the similarities of the other 2 batches were below 0.90.The similarities between the decoction pieces and its ethanol extract were 0.992-0.999 in 430 nm and 0.987-0.999 in 280 nm,which respectively indicates that the traditional alcohol extracting technology of Rhei Radix Et Rhizoma can reserve the main characteristic component.Conclusion:The method is reliable,accurate and reproducible,which can be used for the quality control of the decoction pieces and ethanol extract of Rhei Radix Et Rhizoma as well as for compound preparation containing Rhei Radix Et Rhizoma.
【Fund】: 国家“十一五”科技支撑计划项目(2008BAI53B074);; 广东高校科技成果转化重大项目(cgzhzd1009);; 广东省产学研结合项目(2011B090400319)
【CateGory Index】: O657.72;R284.1
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