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《Chinese Journal of Experimental Traditional Medical Formulae》 2012-22
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Simultaneous Determination of Eight Major Compounds in Mailuoning Injection by HPLC

WU Ting1,ZHANG Jun 1,ZHOU Ling 2,JU Wen-zheng1,TAN Heng-shan3(1.Department of Clinical Pharmacology,Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Nanjing 210009,China; 2.Nanjing University of Traditional Chinese Medicine,Nanjing 210029,China; 3.Department of Clinical Pharmacology,General Hospital of Armed Forces of Nanjing,Nanjing 210002,China)  
Objective:To establish an HPLC method for determination of eight major compounds in Mailuoning injection,including chlorogenic acid,neochlorogenic acid,cryptochlorogenic acid,caffeic acid,cinnamic acid,3,4-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid.Method: The mobile phase was composed of acetonitrile-0.8% acetic acid with gradient elution.The flow rate was 1.0 mL·min-1.The column temperature was set at 30 ℃.The detection wavelength was set at 327 nm for chlorogenic acid,neochlorogenic acid,cryptochlorogenic acid,caffeic acid,3,4-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid and 278 nm for cinnamic acid.The injection volume was 10 μL.Result: The linear ranges of eight compounds were as follows: 2.57-25.7 mg·L-1(r=0.999 7) for chlorogenic acid,2.05-20.5 mg·L-1(r=0.999 7) for neochlorogenic acid,2.26-22.6 mg·L-1(r=0.999 9) for cryptochlorogenic acid,1.92-19.2 mg·L-1(r=0.999 9) for caffeic acid,1.16-11.6 mg·L-1(r=0.999 7) for cinnamic acid,2.282-22.82 mg·L-1(r=0.999 8) for 3,4-dicaffeoylquinic acid,1.839-18.39 mg·L-1(r=0.999 8) for 3,5-dicaffeoylquinic acid and 2.194-21.94 mg·L-1(r=0.999 7) for 4,5-dicaffeoylquinic acid.The average recoveries were 95.26%(RSD 1.21%),96.03%(RSD 1.09%),97.25%(RSD 2.18%),100.98%(RSD 1.98%),97.44%(RSD 2.38%),97.78%(RSD 2.24%),98.37%(RSD 2.15%),95.82%(RSD 2.30%),respectively.Conclusion: The method is convenient,rapid and accurate with good reproducibility and could be used for the quality control of Mailuoning injection.
【Fund】: 江苏省中医药局科学技术研究专项基金(LZ11035);; 江苏省中医药领军人才(LJ200906);; 中药新药临床评价研究技术平台(南京)建设项目(2012ZX09303009-002)
【CateGory Index】: R286.0
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【Citations】
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1 YUAN Bo, LI Lei, LI Fa-mei(School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China);Determination of the contents of chlorogenic acid and caffeic acid in the compound Daqingye Injection by RP-HPLC[J];Journal of Shenyang Pharmaceutical University;2004-02
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