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Content Determination of 3-Hydroxybenzoic Acid in Artemisiae Argyi Folium (Carbonized and Processed with Vinegar) by HPLC

WANG Yong-li;WEI Xiao-hui;ZHAO Sen-miao;LIU Wei;LI Si-meng;CHOU Gui-xin;WANG Zheng-tao;Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine; the MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines; Shanghai R&D Centre for Standardization of Chinese Medicines;  
Objective: To study 3-hydroxybenzoic acid in Artemisiae Argyi Folium(carbonized and processed with vinegar) that come from different arrears,which provide the theoretical basis of the quality control and rational use of Artemisiae Argyi Folium(carbonized and processed with vinegar).Method: HPLC method,the analytical column was Prevail C 18 column(4.6 mm × 250 mm,5 μm).The mobile phase was acetonitrile0.1% phosphate(15 ∶ 85).The flow rate was 1.0 mL·min-1.The detection wavelength was 240 nm and the column temperature was set at 25 ℃.Result: There was good linearity between the peak areas and concentration at the ranges of 0.010 02-0.501 00 μg for 3-hydroxybenzoic acid(r = 1.000 0).The average recovery(n = 9) was 97.39% with RSD 0.99%.Conclusion: The method is accurate,simple and repeatable.The 3hydroxybenzoic acid in Artemisiae Argyi Folium(carbonized and processed with vinegar) of different arrears has significant difference.
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