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《Chinese Journal of Experimental Traditional Medical Formulae》 2013-19
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Optimization of Liquid Fermentative Conditions for Intracellular Polysaccharides from Polyporus umbellatus

XIE Xiu-chao;CHEN Wen-qiang;PENG Hao;DENG Bai-wan;YIN Shu-xue;School of Biological Science and Engineering,Shaanxi University of Technology;Shaanxi Engineering Research Center of Edible and Medicated Fungi;Liuba Edible Fungi Experiment Station;  
Objective: To optimize liquid fermentative conditions of intracellular polysaccharides from Polyporus umbellatus. Method: Fermentative medium for intracellular polysaccharides in P. umbellatus was prepared by shake flask cultivation. With yield of intracellular polysaccharides from P. umbellatus as index,nitrogen source,carbon source and fermentation conditions were investigated by single factor tests,orthogonal test was adopted to optimize composition of culture medium. Result: The best carbon sources was saccharose,optimum nitrogen source was soybean meal,optimized composition of culture medium was as following: potato10. 0%, saccharose 4. 0%, soybean meal 0. 3%, KH2PO40. 2%, MgSO4·7H2O 0. 15%; Fermentation conditions were: initial pH 5. 2-5. 6,shaking speed 180-200 r·min- 1,culture temperature 28 ℃,culture time 7d. Conclusion: It had advantages in fermentation period and polysaccharide production by liquid fermentation,meanwhile it was important to reduce costs and industrial production of polysaccharides from P. umbellatus.
【Fund】: 陕西省教育厅专项科研计划基金项目(08JK247);; 陕西省“13115”科技创新工程计划项目﹙2008IDGC-04﹚
【CateGory Index】: R283
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