Tissue Distribution of Drug-couple Radix Paeoniae Rubra and Rhei Radix et Rhizoma
CHEN Lin;NIU Xiao-yi;ZHANG Zhen-qiu;The Third People's Hospital of Dalian City in Liaoning Province;Liaoning University of Traditional Chinese Medicine;
Objective: To develop an HPLC method for simultaneous quantification of paeonol and rhein in the tissue of rats. Method: The adopted chromatographic column was Phenomsil BDS C18( 4. 6 mm × 250 mm,5 μm),the mobile phase was acetonitrile-0. 1% phosphoric acid aqueous solution( 37∶ 63),the flow rate was 1. 0m L·min-1,and detection wavelength was set at 274 nm. Methyl alcohol was used to precipitate the protein of the plasma samples and the aurantio-obtusin was used as an internal standard. Result: The linear range of paeonol was Y =-0. 055 + 0. 03X( r = 0. 993 2). The intra-day RSD and inter-day RSD were less than 4. 1%. For rhein,the linear range was Y =-0. 152 + 0. 014X( r = 0. 993 5),the intra-day RSD and inter-day RSD were less than4. 9%. The stability of the plasma sample were well acceptable. Conclusion: The developed method for the quantification of paeonol and rhein in rat plasma is suitable for the distribution studies of drug-couple Radix Paeoniae Rubra and Rhei Radix et Rhizoma in the tissue of rats.