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《Chinese Journal of Experimental Traditional Medical Formulae》 2015-21
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Action Mechanism of Shenfu Injection by Computational System Biology Analysis

LYU Yan-ni;FU Long-sheng;ZHOU Jian;CAI Jun;CHEN Xuan-ying;ZHONG Hai-li;Department of Pharmacy,the First Affiliated Hospital of Nanchang University;  
Objective: Shenfu injection is a compound preparation of traditional Chinese medicine,which is comprised of Ginseng Radix et Rhizoma Rubra and Aconiti Lateralis Radix Praeparata. Its main clinical indications of congestive heart failure,ischemic stroke had been widely accepted bymedical community. Based on ischemic stroke,system biology and experimental research was adopted to explain functional mechanisms of Shenfu injection regulating biological network. Method: Departure of 28 compounds in Shenfu injection,Research used combined methods of TCMGene DIT database and Agilent literature search( ALS) system addition to Pharmmapper reverse docking to dig the protein information of Shenfu injection to biuld multi-compounds protein network. Protein interaction network was built based on protein-protein interaction extracted from Genecards, BIND, Bio GRID,Int Act,Mint database. Extracted significant difference subnetwrok from the netwrok and subjected it to Western blot verification. Result: The whole 28 compounds had connections with 55 proteins,while protein interaction network was built with none-isolated nodes of 53 proteins,from which one subnetwork was extracted with significant difference. Fifteen key proteins in subnetwork were mapped on Biocart signaling pathway,which had relationship with NF-κB signaling pathway,AKT signaling pathway,Toll signaling pathway,MAPK signaling pathway,etc.Western blot confirmed Shenfu injection could attenuate phosphorylation expression of NF-κB p65, IκB-α on ischemia 1 h reperfusion 24 h MCAO model. Conclusion: Computational system biology and experimental study approach preliminary explores molecular mechanism of Shenfu injection for preventing and curing diseases,it can provide references for researches on Chinese medicines.
【Fund】: 江西省卫生厅课题(20155144)
【CateGory Index】: R285
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