Effects of Arisaematis Rhizoma Polysaccharide on Proliferation and Apoptosis of Human Renal Cell Carcinoma Cell Line GRC-1
TANG Hua-yong;ZHANG Wan-sheng;YU Hang;JIANG Shuang;WANG Li-guo;Affiliated Hospital of Jilin Medical University;
Objective: To explore Arisaematis Rhizoma polysaccharide( ARPS) on the proliferation,apoptosis and Wnt/β-catenin signaling pathway of human renal cell carcinoma cell line GRC-1. Method: After entering into the logarithmic growth phase with RPMI-1640 culture fluid under routine condition,the GRC-1 cells were treated with different doses of ARPS( 0,20,50,100,200 mg·L~(-1)). The 0 mg·L~(-1)ARPS group was the blank group. The inhibition rates of proliferation at 24,48,72 and 96 h after the treatment were measured by viable cell counting kit assay( CCK-8). The apoptosis rate and cell cycle at 48 h after the treatment were detected by Annexin-FITC/PI double staining and PI staining methods via flow cytometry,respectively. Western blot was employed to detect the expression level of β-catenin in Wnt/β-catenin signaling pathway and its downstream target molecule C-myc and Cyclin D1 at 48 h after the treatment. Result: Compared with the blank group,ARPS within the range of 20-200 mg·L~(-1)presented an inhibitory effect on GRC-1 cell proliferation. The proliferation inhibition rate increased with the rise in time and concentration of ARPS in a dose-and time-dependent manner,and the above differences were statistically significant( P 0. 05). Compared with the 0 μg·m L~(-1)ARPS,the early apoptosis rate of 50-200 mg·L~(-1)ARPS,the late apoptosis rate of 20-200 mg·L~(-1)and the total apoptosis rate were all elevated( P 0. 05). There was a higher G_0/G_1 phase ratio than 20,50,100,200 mg·L~(-1)ARPS groups,and lower S and G2/M phase and β-catenin,C-myc and Cyclin D1 protein levels than 0 mg·L~(-1)( P 0. 05). Indexes within the concentration range between 20-200 mg·L~(-1)were statistically significant( P 0. 05). Conclusion: ARPS inhibited the proliferation of renal cell carcinoma cell line GRC-1,and induced cell apoptosis and G_0/G_1 arrest,with the effect of inhibiting the activation of Wnt/β-catenin signaling.
【Fund】： 吉林省教育厅"十二五科学技术研究项目"(吉教科合字2014203);; 吉林省中医药管理局中医药科技项目(2012163)
【CateGory Index】： R285
【CateGory Index】： R285