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《Chinese Journal of Experimental Traditional Medical Formulae》 2017-01
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Protective Effect and Mechanism of Danlou Tablets on Myocardial Ischemia Reperfusion Injury in Rats

LI Jun-ping;GUO Li-li;CHEN Zhong;WEI Ben-jun;YUAN Rong;CHEN Heng-wen;WANG Jie;Guang'anmen Hospital,China Academy of Chinese Medical Sciences;Hubei University of Chinese Medicine;  
Objective: To explore the protective effect and mechanism of Danlou tablets(DT) on myocardial ischemia reperfusion injury(MIRI) in rats. Method: The 80 male healthy Wistar rats with body weight of(300 ± 20) g were randomly divided into sham operation group,model group and Danlou tablets group.Rats in sham operation group and model group were administered with 0. 5% CMC-Na solution at the daily dose of10 m L·kg~(-1)by ig,and rats in the DT group were administered with 0. 9 g·kg~(-1)Danlou tablets. After prophylactic administration for 7 days,the left anterior descending branch of the rats heart were ligatured for 50 minutes to create the models of myocardial ischemia reperfusion injury in rats. The levels of lactate dehydrogenase(LDH),creatine kinase isoenzyme(CK-MB),endothelin(ET) and nitric oxide(NO) in serum,and malondialdehyde(MDA) and myeloperoxidase(MPO) in myocardial tissues were detected after 2 h reperfusion in 6 rats of each group. Ultrasound was used to observe the cardiac ejection fraction(EF) before and after 48 h reperfusion. The 6rats in each group were selected for Td T-mediated d UTP nick end labeling(TUNEL) staining to assess the apoptosis index of myocardial cells,and then the protein expression levels of Caspase-3,Bax and Bcl-2 protein in cardiac muscle tissues were detected by Western blot. Evans blue-TTC staining was used to assess the myocardial infarction area in the remaining rats. Result: After reperfusion for 2 h,as compared with model group,the levels of serum LDH,CK-MB and ET-1 in DT group were significantly reduced(P 0. 05),while the levels of NO,MDA and MPO were significantly elevated(P 0. 05). After reperfusion for 48 h,as compared with model group,the EF values in Danlou tablets group were significantly elevated(P 0. 05),with obvious decreases in the myocardial infarction size and myocardial apoptosis index(P 0. 05),myocardial Caspase-3 and Bax protein expression levels in Danlou tablets group were significantly increased(P 0. 05,P 0. 01),and Bcl-2 protein expression levels were decreased(P 0. 05). Conclusion: Danlou tablets can protect endothelial cells,inhibit oxidative and inflammatory infiltration in the early stage of MIRI. The protective effect on myocardial ischemia reperfusion injury in rats of Danlou tablets may be related to inhibiting apoptosis,reducing myocardial infarct size and improving cardiac function in the late stage of MIRI.
【Fund】: 国家“重大新药创制”科技重大专项(2013ZX09301307)
【CateGory Index】: R285.5
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