Analysis on Chemical Constituents of Glycyrrhizae Radix et Rhizoma in Pinelliae Rhizoma Praeparatum by UPLC-Q-TOF-MS/MS
YANG Bin;WANG Yuan;TIAN Meng;YUAN Lei;WU Yuan-yuan;LI Yu-bo;College of Chinese Materia Medica,Tianjin University of Traditional Chinese Medicine;
Objective: To analyze the chemical components of Glycyrrhizae Radix et Rhizoma in Pinelliae Rhizoma Praeparatum(PRP) by using ultra performance liquid chromatography-quadrupole-time of flightmass spectrometry(UPLC-Q-TOF-MS/MS). Method: The aqueous extracts of PRP were separated on Waters Acquity BEH C18column(2. 1 mm × 100 mm,1. 7 μm) with acetonitrile-0. 1% formic acid solution as mobile phase for gradient elution. The flow rate was 0. 25 m L·min-1and the column temperature was 35 ℃. Mass spectrometric data were obtained under electrospray ionization(ESI) negative mode and the constituents were identified according to the exact relative molecular weight and fragment ions reported in the references. Result:Fifteen constituents were identified from Glycyrrhizae Radix et Rhizoma in PRP,including 6 triterpenoid saponins and 9 flavonoids. Conclusion: Systematic research of the fifteen chemical constituents of Glycyrrhizae Radix et Rhizoma in PRP was performed firstly in this study,which could provide the basis for clarifying the pharmacodynamic material basis and rational clinical application of PRP.