Gastrointestinal Absorption Kinetics of Polydatin and Resveratrol from Polygoni Cuspidati Rhizoma et Radix Extract in Rats
OU Shui-ping;REN Li;WANG Yu-he;WANG Sen;CHEN Ling;Department of Pharmacy,Affiliated Hospital of Zunyi Medical University;School of Pharmacy,Zunyi Medical University;
Objective: To study on the absorption characteristics of polydatin and resveratrol from Polygoni Cuspidati Rhizoma et Radix extract in the stomach and small intestine of rats. Method: In situ single pass intestinal perfusion model in rats was adopted,taking contents of polydatin and resveratrol as indexes,HPLC was employed to determine contents of polydatin and resveratrol before and after being perfused Polygoni Cuspidati Rhizoma et Radix extract for investigating their absorption characteristics in the stomach and small intestine of rats.Result: The absorption rate of Polygoni Cuspidati Rhizoma et Radix extract was proportional to drug concentration in the stomach. Absorption rate of polydatin in Polygoni Cuspidati Rhizoma et Radix extract with low dose showed significant difference(P 0. 05) when compared with the middle and high dose groups, but there was no significant difference between the middle dose group and the high dose group. The absorption rates of resveratrol at different dose groups indicated significant deviation by SPSS 17. 0(F = 609. 724,P 0. 05). When the dose of Polygoni Cuspidati Rhizoma et Radix extract was 600 mg·L~(-1),the absorption rate constants(Ka) of polydatin and resveratrol were 0. 925,0. 575 h-1,respectively. The Kaand absorption rates of polydatin and resveratrol in different dose groups were not significantly different. Conclusion: Polydatin in Polygoni Cuspidati Rhizoma et Radix extract has a concentration saturation phenomenon in the stomach, its mechanism may be an active absorption or facilitated diffusion; while the absorption rate of resveratrol in the stomach increased with the increase of extract concentration,this suggests that the resveratrol might be absorbed in the stomach via passive transport mechanism. Different concentrations of Polygoni Cuspidati Rhizoma et Radix extract had no effect on the intestinal absorption of polydatin and resveratrol,and absorption is not saturated,this suggests that polydatin and resveratrol may be absorbed in intestine via passive diffusion mechanism.
【Fund】： 贵州省科技合作计划项目(黔科合LH字7548号 7481号);; 遵义市科技计划课题(遵市科合社字96号)
【CateGory Index】： R285.5
【CateGory Index】： R285.5