Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)》 2017-06
Add to Favorite Get Latest Update

Relationship between the m RNA transcription levels of AcrAB-TolC efflux pump in Shigella from clinical source and fluoroquinolone resistance

Liu Feng Yun;Zhang Haojie;Yang Xian;Wu Xiaomei;Qi Wei;Institute of Infectious Diseases, The Second Hospital of Tianjin Medical Univercity;  
Objective To investigate the relationship between the transcriptional levels of messenger RNA(m RNA) from component genes(acr A, acr B, tol C) of the efflux pump Acr AB-Tol C of clinical isolates of Shigella flexneri and the level of fluoroquinolone resistance. Methods Total of 192 clinical Shigellaisolates were collected from non-repetitive faeces of diarrhea patients during 2009 to 2015 from 3 grand three hospitals in Tianjin, which were The General Hospital of Tianjin Medical Univercity, The Second Hospital of Tianjin Medical Univercity and The Children's Hospital in Tianjin. Antibiotic susceptibility of the Shigella clinical isolates were detected by K-B disk diffusion. The strains were divided into the fluoroquinolone resistant group and full fluoroquinolone sensitive group, according to susceptibility to fluoroquinolone. The m RNA transcription of Acr AB-Tol C efflux pump of the two groups of strains determined by real-time quantitative PCR(RT-PCR). And the SPSS 17.0 software was used for statistical analysis. Results There were 9 isolates in fluoroquinolone resistance group, which were resistant to three and above kinds of fluoroquinolone. And the number in the fluoroquinolone resistance group were F2, N8, F44, 157, 187, 368, 1113, 3171 and 3327. While there were 9 isolates in fluoroquinolone full-sensitive group. And the number were F13, 1, 22, 174, 186, 377, 1506, 3283 and 3326. The m RNA transcription level of acr A in the resistant group was higher than that of the full-sensitive group, with significant difference(t =2.97, P = 0.02). Although the m RNA transcription levels of acr B and tol C in the resistant group were higher than those of the full-sensitive group, but with no significant difference(t = 2.84, P = 0.30; t = 1.17, P = 0.29). Conclusions There was a certain correlation between the high m RNA transcription level of acr A and the resistance of fluoroquinolones in Shigella.
【Fund】: 天津市卫生局科技基金项目(No.201440296)
【CateGory Index】: R446.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved