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《Chinese Journal of Nervous and Mental Diseases》 2010-01
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The effect of hyperglycemia on the expression of glucose transporter 1 in rats following traumatic brain injury

WANG Liang,TANG Wenyuan,SUNG Xiaochuan,DENG Luping,LI Dong. Department of Neurosurgery,First Affiliated Hospital,Chongqing Medical University,1 Yixueyuan Road,Chonqing 400016,China.  
Objective Aim to explore the effect of hyperglycemia on the expression of glucose transporter 1 (GLUT-1)in rats at the acute phase of traumatic brain injury. Methods Adult male SD rats were randomly divided into 3 groups: normal control group,traumatic brain injury group and insulin treated group. The blood glucose concentration of the rats was measured before and after injury. The expression of GLUT-1 gene and protein in the injured and uninjured cortex was detected by reverse transcription polymerase chain reaction (RT-PCR) and western-blot. The apoptosis in the injured and uninjured cortex were detected by TUNEL staining. Results The blood glucose concentration increased markedly in traumatic brain injury group. The expression of GLUT-1 gene and protein in the injured cortex decreased in both the traumatic brain injury group and the insulin treated group. In contrast,the expression of GLUT-1 gene and protein significantly increased at 12 h,24 h,48 h,72 h after injury in the insulin treated group compared with control(P0.01). The number of apoptotic cells in the insulin treated group were significantly larger than that in the traumatic brain injury group at each time point(P0.01). However,The number of apoptotic cell death remained unchange in the uninjured cortex in each group(P0.05). Conclusions The hyperglycemia after traumatic brain injury may increase the apoptotic cells in the injured brain through decreasing the expression of GLUT-1.
【CateGory Index】: R651.1
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 LIU Yuan, WANG Li, ZENG Lin, et al . Research Institute of Field Surgery, Chongqing 400042, China;Establishment of Model of Traumatic Brain Injury by an Improved Method in Rats[J];Chinese Journal of Clinical Neurosurgery;2008-07
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