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Production and Preliminary Usage of Polyclonal Antibody against Ciliary Neurotrophic Factor in Immunocytochemical Studies

Yang Qingfeng; Xian Haiqing ;Tan Xiangling; Cao Zheng ;Wan Xuancai; et al(Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850)  
Anti-hCNTF polyclonal antibody was purified from the sera of the rabbits immunized byrecombinant human ciliary neurotrophic factor (hCNTF) by precipitation of ammonia sulfateand protein A affinity chromatography. The results of immunohistochemical staining withthe antibody showed that CNTF substance was located at neurons in all layers of cerebraland cerebellar cortex, pyramidal cells in hippocampus, granular cells in dentate gyrus, aswell as in neuron nucleus of facial nerve besides glial cells in these parts. Otherwise, CNTFsubstance was located not only in cytoplasm but in nucleus of neurons as well.
【Fund】: 国家“863”项目!(94)国科生字106号
【CateGory Index】: R392
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