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《Chinese Journal of Food Hygiene》 2014-04
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Survey and hemolysin gene analysis of Bacillus cereus in infant food fom Shijiazhuang market

QIN Li-yun;LV Guo-ping;GUO Yu-mei;QI Hui-rong;PAN Zhuo;Shijiazhuang Center for Diserse Control and Prevention;  
Objective To investigate the contamination of Bacillus cereus in infant formula milk powder and cereal-based complementary foods from Shijiazhuang market,and also to monitor the toxin genes of the isolated strains. Methods Infant formula powder and cereal-based complementary foods from 23 counties of Shijiazhuang city were collected. Bacilluscereus strains were tested and counted according to the national standard GB /T 4789. 14-2003 and foodborne pathogenic bacteria monitoring manual. The hemolytic toxin genes and non-hemolytic toxin genes of isolates were detected by real-time PCR. Results 85 strains of Bacillus cereus were detected from 399 samples. The detection rate of infant formula powde was 20. 3%( 36 /158) and cereal-based complemenary foods was 22. 8%( 49 /241). The hemolysin gene was positive in48 isolated strains and the detection rate was 56. 5% with no isolates carrying non-hemolytic toxin gene. Conclusion The contamination of Bacillus cereus in infant food was quite severe. These foods had a potential food poisoning risk. Our results could provide reference for the infant food hygiene inspection standard setting,supervision and management.
【Fund】: 石家庄市科学技术研究与发展指导计划(121461203)
【CateGory Index】: R155.3
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