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《Chinese Journal of Food Hygiene》 2014-06
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Investigation of Bacillus cereuse of toxin gene and metic toxin in infant milk powder and rice flour

ZHANG Le-yi;ZHANG Xiu-yao;LI Yi;CAI Xin-xin;WANG Xin;Wenzhou Center for Disease Control and Prevention;  
Objective To understand the Bacillus cereus pollution occupation in infant formula and rice flour,and learn the carriage trait of emetic toxin、toxin gene of Bacillus cereus. Methods The dilution culture count method( MPN counting)was used to isolate Bacillus cereus. PCR was to analyze the toxin gene type,including diarrhea toxin genes and enterotoxin genes and ultra performance liquid chromatography-triple quadrupole mass spectrometry( UPLC-MS / MS) was to detect the emetic toxin-Cereulide. Results 28 Bacillus cereus strains were detected in 39 samples,detection rate was 71. 79%( 28 /39),and two samples( 5. 13%) carried the emetic toxin. All the strains carried at least three toxin genes revealing most of them are strains of multiple toxic genes. Besides that,nhe ABC and ent FM genes were the common shared toxin genes. The detection rate of nhe ABC genes and ent FM were 100%,35. 71% respectively. cyt K gene was the least frequently detected gene in isolated strains. Conclusion It should be maintained that the surveillance of contamination occurrence of Bacillus cereus in infant milk powder and rice flour and the study of pathogenicity of Bacillus cereus to evaluate the possible threat against infant food safety from Bacillus cereus scientifically and practically.
【Fund】: 温州市2013年科技项目(Y20130209)
【CateGory Index】: R155.5
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