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《Chinese Journal of Food Hygiene》 2015-04
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Distribution of virulence genes and virulence evaluation of food-borne Bacillus cereus in Shijiazhuang City

QIN Li-yun;LV Guo-ping;CAI Zhen;PAN Zhuo;QI Li-rong;Shijiazhuang Center for Disease Control and Prevention;  
Objective To study the distribution of virulence genes,evaluate the virulence of food-borne Bacillus cereus in Shijiazhuang City,and to investigate the potential risk. Methods 9 virulence genes( such as enterotoxin and voitoxin)were detected with PCR method in 131 Bacillus cereus collected from food risk monitoring program. The virulence of pathogens were also analyzed using blood agar plates. Results The carrying rate of virulence genes was high. 99. 2%( 130 /131) of the strains carried at least one virulence gene. hbl ACD and ent FM were the major virulence genes of Bacillus cereus in Shijiazhuang City. All strains produced hemolysin BL. Conclusion Diarrhea enterotoxin was widespread in food,and hemolysin was detected in all pathogens,which caused potential public health risk. More effective monitoring strategies should be developed to prevent and control the occurrence of Bacillus cereus food-borne diseases.
【Fund】: 石家庄市科学技术研究与发展指导计划(121461203)
【CateGory Index】: R155.5
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