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《China Practical Medicine》 2010-03
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The effect of ERK signaling cascade pathways on the inhibition of Berberine on lipopolysaccharide induced COX-2 expression

WANG Qi-zhang,GUO Yi,HAN Li-min,et al. Department of Neurology,Shenzhen Shajing Hospital ,the Afffliated Hospital of Guangzhou Medical College,Shenzhen 518104,China  
Objective To investigate the inhibition of berberine (BBR) on lipopolysaccharide(LPS) induced COX-2 expression via ERK signaling cascade pathways in human peripheral blood monocytes (HPMC). Methods HPMC were isolated and cultured from whole blood and divided into 5 groups treated with null,LPS,LPS + BBR 25 μmol/L,LPS + BBR 50 μmol/L,LPS + BBR 100 μmol/L respectively for 30 minutes,6 hours,12 hours and 24 hours. Then monocytes were extracted for RT-PCR and westernblot analysis to examine COX-2 mRNA and protein activated expression of ERK signaling pathway. Meanwhile,PD98059 (ERK inhibitor) was incubated together with LPS stimulation to examine COX-2 mRNA and protein expression. Results At the four time points after treatment,the COX-2 mRNA and protein expression decreased at a low ebb at 12 hours after BBR treatment(P 0. 05). On the other hand,with the increasing concentration of BBR, the COX-2 expression decreased progressively (P 0. 01). In the group of BBR treatment at various time points (at 6 h,12 h,24 h after treatment) and three levels of dosage,ERK protein expression was inhibited significantly. At 12 hours after BBR administration the ERK protein expression reduced more prominently than that of other three time points ( P 0. 05),and ERK protein expression decreased progressively as the dosage increased. Human monocytes COX-2 mRNA and protein expression was inhibited significantly while incubated with ERK pathway inhibitor,PD98059 (P 0. 01). Conclusion It is concluded that BBR inhibits COX-2 mRNA and protein expression in a dose-dependent manner and ERK expression can be suppressed by BBR. BBR inhibits COX-2 expression via ERK signaling pathway in HPMC.
【CateGory Index】: R285
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