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《China Food Additives》 2012-04
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Studies on the extraction and purification of total flavones in camellia oleifera abel leaves

XIAO Xin-sheng,ZHANG Min,YUAN Xian-you,LI Dong,YANG Ying(Department of Chemistry and Living Creature Engineering,Hunan University of Science and Engineering,Yongzhou 425100)  
The extraction of flavones from Camellia oleifera abel leaves by ultrasonic wave was studied.The results provided scientific basis for industrial production of the functional flavonoids from Camellia oleifera abel leaves.By single factor and orthogonal experiments,the optimum conditions of ultrasonic wave power,alcohol concentration,extraction time and the ratio of solid and liquid were determined.The optimal conditions were:60% alcohol,ultrasonic wave power at 450W,extraction time 30min and the ratio of solid and liquid 1 ∶ 20.Under the above conditions,the content of flavonoids in the old leaf the content of flavonoids in the middle leaf the content of flavonoids in the young leaf.AB-8 resin was used to separate and purify the total flavones.Sample flow rate was 3BV/h,the solution concentration was 2.0mg/mL for absorption,3BV 90% ethanol and 1.5BV/h of elution was the flow rate for desorption.The purity of flavonoids was increased from 13.1% to 27.2%.
【Fund】: 国家社会科学基金重点项目(11AGL004);; 湖南省“十二五”重点建设学科(湘教发[2011]76号);; 湖南省科技计划项目(2010NK3007);; 永州市科技计划项目(2011-6)
【CateGory Index】: TQ461
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