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Effects of Vitamin D_3 on the Activitiy of Three Antioxidant Enzymes in Ruditapes philippinarum

HE Liang-hua,CHEN Qiang,GAN Ping-xu,WANG Shou-kun,CHEN Jian-ye(College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)  
The present study examined the effects of vitamin D3 on three antioxidant enzymes in visceral mass of R.philippinarum.The three concentrations of vitamin D3 were 0(control),5000,10000,15000 and 20000 International Units of vitamin D3 per litre of seawater(IU/L) in the 96-hour experimental bath treatment.The catalase(CAT),superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) of visceral mass were assayed respectively.The results indicated that the activity of CAT and SOD in visceral mass of R.philippinarum increased significantlyin 48h and declined significantly with falling tendency after 48h(p0.05).The activity in 10000(IU/ L) group was higher than others(p0.05).The activity of GSH-Px increased significantly in 96h with rising tendency.The study showed that the activity of the three antioxidant enzymes were increased with the treatment of vitamin D3 which dose was better from 10000 to 15000(IU/L).
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