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《Edible Fungi of China》 2002-02
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Studies of Technology of Isolation and Purification of Lentinan

WANG Wei-guo 1,ZHAO Yong-liang 2,HAN Shan-bao 3 (1.Dept.of Biochemical Engineering,Nanyang Institute of Science and Technology Nanyang Henan 473004;2.Crop′s Genetic and Breeding Dept.,Sichuan Agricultural University,Ya′an,Sichuan,625014,China;3.Bi  
The optimum conditions of isolation and purification for lentinan were studied comparatively.The results showed that the optimal technological conditions of separation and purification of lentinan are:the size of Lentinus edodes particles 2~3mm in diameter,Lentinus edodes/water 1∶20,distilling at 96℃ for hrs,precipitated by ethanol at 70%.The best method is method BECM while depriving dregs of proteins;the deproteinization rate is up to 99 98% by BECM.Three ingredients L1,L2 and L3 were acquired by grade centrifuge with methanol.the content of polyglucosan or glucan of L1,L2 and L3 were acquired by grade centrifuge with mathanol.The content of polyglucosan or glucan of L1,L2 and L3 were 68 09%,79 25% and 100% respectively by the methods of acidolysis,chromatography on paper and phenolsulfuric acid.The lyophilization or freeze-drying method should be an ideal way while drying pure active biochemical products compared with others methods such as natural drying,high temperature and so on.
【Fund】: 南阳市科委攻关项目
【CateGory Index】: O636
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