Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《ACADEMIC JOURNAL OF SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES》 2000-02
Add to Favorite Get Latest Update

The Investigation of Cell Culture Conditions Maintained Embryonic Stem Cells as Totipotent Cells

QIU Guan ting 1, HUANG Bing 2, TANG Shi bo 1, HU Jie 1, LIU Zhi xiong 1 (1. Vitreoretinal Laboratory, Zhongshan Ophthalmic Center, Guangzhou, 510060; 2. Experimental Animal Center, Sun Yat sen University of Medical Sciences, Guangzhou 510089,  
Objective To investigate the culture conditions of embryonic stem(ES) cells which can retain their totipotency. Method ES D3 cells were cultured in the presence of feeder layer cells including SNL cell line and primary mouse embryonic fibroblast (PMEF) and in the absence of feeder layer provided the medium supplemented with purified murine leukemia inhibitory factor(mLIF) or buffalo rat liver (BRL) condition media respectively. The alkaline phosphatase (AKP) test and karyotype analysis were carried out. Results ES D3 could develop normal clone on the above mentioned cell culture systems. Moreover, ES D3 cells within the colonies continued to express AKP over many passages and keep their normal karyotype. Conclusions It indicated that above mentioned culture systems could effectively maintain ES D3 cells undifferentiated state and normal diploid karyotype.
【Key Words】: embryonic stem cells culture media conditioned
【Fund】: 国家自然科学基金!( 3 9770 790 )
【CateGory Index】: R321
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 6 Hits
1 WU Xuan, XU Hai wei, LI Hai di, FAN Xiao tang, CAO Juan, TANG Jun (Department of Physiology, Third Military Medical University, Chongqing 400038, China);AANAT gene-engineered embryonic stem cells decrease the amyloid β secretion of APP-overexpressed PC12 cells[J];Acta Academiae Medicinae Militaris Tertiae;2003-03
2 Chun-Hua Meng,Chuan-Sheng Zhang1,Na-Na Yang2,Zi-Yu Wang3,Shao-Xian Cao,Li-Xin Du4 (Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China; 1Department of Animal Science,Hebei Normal University of Science and Technology,Changli 066600,China; 2Insititute of Life Science,Taishan Medicine University,Tai’an 271000,China; 3College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China; 4Institute of Animal,Chinese Academy of Agricultural Sciences,Beijing 100094,China);Preliminary Study on Culture of Chicken Embryonic Stem Cells by Buffalo Rat Liver Cells Conditioned Medium[J];Chinese Journal of Cell Biology;2008-04
3 Wang Ying1,2,Yue Wenbin2,Chen Lina1,Zhang Yanyan3,Guan Weijun1** (1.Institute of Animal Science,Chinese Academy of Agricultural Science,Beijiang 100094; 2.Shanxi Agricultural University,Taigu,Shanxi 030801; 3.Northwestern Sci-tech University of Agriculture and Forestry,Yangling,Shaanxi 712100);Effect Factors of Isolation & Culture and Identification of Cell Biological Characteristics of Poultry Embryonic Stem Cells[J];China Poultry;2006-20
4 Dan-yah ZHU Yi-jia LOU~2 Department of Pharmacology and Toxicology,College of Pharmaceutical Scienees,Zhejiang University,Hangzhou 310031,China;Inducible effects of icariin,icaritin,and desmethylicaritin on directional differentiation of embryonic stem cells into cardiomyocytes in vitro[J];中国药理学报(英文版);2005-04
5 TAG Jing, GE Jian, HUANG Bing, ZHUO Ye-hong, CHEN Hui-yi, GUO Yan, CHEN Xi-gu (1. Zhongshan Ophthalmic Center, 2. Experimental Animal Center, SUN Yat-sen University, Guangzhou 510060, China);Comparative Study on Culture and Differentiation of Neural Stem Cells Derived from Embryonic Stem Cells and Hippocampus[J];Journal of Sun Yat-sen University (Medical Sciences);2003-03
6 SANG Run zi,HAN Jian yong,SUN Guo jie,JIN Dong hang,TIAN Shu jun (College of Animal Science and Technonlogy,Agricultural University of Hebei,Baoding,Hebei 071001,China);Isolation and Cloning of ES-like Cells of Goat[J];Chinese Journal of Veterinary;2003-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Zhu Zhi, Zhu Ming Hua Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, P.R.China;Research Advances on p53 Gene Network[J];Chinese Journal of Cancer;2003-05
2 LlU RONGXIU(Department of Animal Science and Veterinary Medicine, Shenyang Agricultural University);ON THE GENESIS OF PRIMORDIAL GERM CELLS (PGCs) IN AVES: Ⅰ. ULTRASTRUCTURAL OBSERVATIONS ON THE PGCs IN THE GERMINAL CRESCENT AREA OF THE CHICK EMBRYO[J];Acta Zoologica Sinica;1990-02
3 LI Zan Dong ① LIU Chun Hai ① HUANG Jin Song ① SHA Jin ① WEI Hua ① SUN Ming Jun ① ZHAO Chen ① JIANG Sheng Hua ① Hiroshi KAGAMI ② (①College of Biological Sciences, China Agricultural University, Beijing 100094, China) (②;PREPARATION OF INTERSPECIES CHICKEN (GALLUS DOMESTICUS)/DUCK (ANAS DOMESTICUS) CHIMERAS[J];Acta Zoologica Sinica;2002-04
4 An Jing ① Du Li Xin ②** (① College of Animal Science and Technology, Shandong Agriculture University, Taian 271018, China) (② Institute of Animal Science,CASS, Beijing\ 100094,China);Isolation,cultivation and identification of chicken embryonic stem cells[J];Acta Zoologica Sinica;2003-05
5 SHANG Kegang, HU Xinli (Department of Biology, Peking University);The Effects of Different Genetic Background of Mouse Blastocysts on the Formation of Embryonic Stem Cell Colonies[J];Acta Scicentiarum Naturalum Universitis Pekinesis;1993-02
6 HU Xinli;SHANG Kegang (College of Life Science,Peking University,Beijing,100871);Establishment and Characterization of Six ES Cell Lines from Mouse 129/ter Strain[J];Acta Scicentiarum Naturalum Universitis Pekinesis;1996-02
7 TONG Ying\ ZOU Jizhong\ SHANG Kegang (College of life sciences, Peking University, Beijing, 100871);Establishment of a C57BL/6J ES Cell Line by Conditioned Media of Rat Myocardial Cells[J];ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS;2000-04
8 SHANG Kegang HU Xinli LI Ziyu WANG Xueqing LIU Aimin MENG Guoliang TONG Ying(Department of Biology,Peking University,Beijing,100871);Effects of Different Feeder Layers on the Establishment and Character Maintenance of Mouse ES Cell Lines[J];ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS;1994-04
9 HAN Rong\ CHAI Guixuan\ SHANG Kegang (College of Life Sciences, Peking University, Beijing, 100871);The Effect of Rat Heart Conditioned Medium in Forming Mouse ES Cell Colonies[J];ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS;1997-02
10 TONG Ying LIU Aimin SHANG Kegang (College of Life Sciences, Peking University, Beijing, 100871) LIU\ Lin (Department of Animal Science, Beijing Agricultural University, Beijing, 100094);Establishment and Characterization of Rabbit ES like Cell Lines[J];ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS;1997-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 YANG Li-fen et al (College of Animal Science,Henan Institute of Science and Technology,Xinxiang,Henan 453003);Research Advance in Isolation and Culture of Embryonic Stem Cells in Mammalian[J];Journal of Anhui Agricultural Sciences;2006-12
2 ;Comparison of methods for culturing mouse embryonic stem cell line ES/GFP and its effect on cell proliferation and differentiation[J];Acta Academiae Medicinae Militaris Tertiae;2004-19
3 LI Da-bing 1 , TANG Jun 1 ,FAN Xiao-tang 2 ,SONG Min 3 ,XU Hai-wei 1 ,ZHOU Guang-ji 1 ,BAI Yun 3 ( 1 Department of Physiology, 2 Department of Neurobiology, 3 Department of Medical Genetics,College of Medicine,Third Military Medical University,Chongqing 400038,China);Histopathologic comparison between PS1/APP double transgenic mouse model and Aβ_(1-40)-injected rat model of Alzheimer disease[J];Acta Academiae Medicinae Militaris Tertiae;2006-16
4 WANG Ying1,2,CHEN Li-na1,ZHANG Yan-yan3,GUAN Wei-jun1(1.Institute of Animal Science,Chinese Academy of Agriculture Science,Beijing 100094,China;2.Shanxi Agricultural University,Taigu 030801,China;3.Northwestern Sci-tech University of Agriculture and Forestry,Yangling 712100,China);Effects on Embryonic Stem Cells and Influencing Factors of Isolation & Culture of Feeder Cells[J];China Animal Husbandry & Veterinary Medicine;2007-11
5 PAN Ying,LIU Yan-yan,CHU Ning-ning,SONG Xue-xiong(College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China);The Effects of 17β-estradiol on Culture of Chicken Embryonic Primordial Germ Cells in Vitro[J];China Animal Husbandry & Veterinary Medicine;2009-01
6 GE Xiu-guo et al(Department of Animal Science,Tianjin College of Agronomy,Tianjin 300384);Isolated Culture of Nuclear Transplantation Embryonic Stem Cells of Goat[J];Journal of Anhui Agricultural Sciences;2011-07
7 ZHU Jing1,DOU Ru-hai1,JIN Dong-qing1,YANG Na-na2(1.Shandong Provincial Center of Laboratory Animals,Jinan 250002,China;2.Taishan Medical University,Tai'an 271016,China);Construction of Eukaryotic Expression Vector of pSecTag-EGFP and Research of Chicken Embryonic Fibroblast Cells[J];Laboratory Animal and Comparative Medicine;2008-05
8 Long Yan, Lei Lei, Chunrong Yang, Zhimin Gao, Anmin Lei, Xiaoling Ma, and Zhongying Dou Shaanxi Key Laboratory for Molecular Biology of Agriculture, Shaanxi Center of Stem Cell Engineering & Technology, Northwest A & F University, Yangling 712100, China;Isolation and Cultivation of Goat Embryo Stem Cells[J];Chinese Journal of Biotechnology;2008-09
9 Zhang Yanyan1,2 Wang Ying1,3 Chen Lina1 Li Linfeng1,4 Jin Yaping2 Guan Weijun1 Ma Yuehui1(1Institute of Animal Science,Chinese Academy of Agricultural Science,Beijing 100094;2College of Animal Medicine,Northwest Sci-Tech University of Agriculture and Forestry,Yangling 712100;3College of Animal Science,Zhejiang University,Hangzhou 310029;4College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030);Researches on Avian Embryonic Germ Cells[J];Biotechnology Bulletin;2008-06
10 LIANG He,WANG Jia,CAO Nan,HUANG Ji-jun,YANG Huang-tian*(Key Laboratory of Stem Cell Biology,Institute of Health Science,Shanghai Institutes for Biological Sciences/Shanghai Jiaotong University School of Medicine,Shanghai 200025,China);Differentiation of embryonic stem cells and induced pluripotent stem cells into cardiomyocytes:progresses and perspectives[J];Chinese Bulletin of Life Sciences;2009-05
China Proceedings of conference Full-text Database 1 Hits
1 An lilong,XiaoMei Zhang TuBao,Dong XiFang,Xu YingMei(college of Agriculture,GuangDong Ocean University,Zhanjiang Guangdong 524008,China);Effect of Different Concentration Neonatal Bovine Serum on the Growth and Proliferation of Primordial Germ Cells(PGCs) from Three-Yellow Broilers[A];[C];2008
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved