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Zhou Yuanping 1 Peng Wenwei 1 Yao Jilu 1 Zhang Zhigang 2 Luo Guanghan 3 Deng Zhide 1 Gu Xiuhua 1 (1 Department of Infectious Diseases, 2 Pathology, Third Affiliated Hospital, Sun Yat sen University of Medical Sciences, Guangzhou, 510630,  
In order to localize a trace of HCV RNA in tissue sections from patients with hepatocellular carcinoma (HCC), a technique of in situ polymerase chain reaction was developed and carried out in paraffin embedded sections of cancerous and pericancerous liver tissues from 17 HCC patients. The results showed that the specificity of the technique was satisfactory. The positive rates of HCV RNA in sections of cancerous and pericancerous liver tissues were 41.2% and 70.6% respectively. The positive signals mainly localized in cytoplasm. But in cancerous tissues, the positive signals in nuclei and nuclear membrane were significantly increased. These results further support the association between HCC and HCV infection and suggest the interaction between HCV gene and host genome.
【Fund】: 国家教委博士点基金 CMB资助
【CateGory Index】: R512.6
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Chinese Journal Full-text Database 1 Hits
1 LI Ning, YAO Yingmin. Department of Pediatrics,Nanfang Hospital, the First Military Medical University, Guangzhou 510515, China;Multi-system rotavirus infection in 2 infants[J];Chinexe Journal of Pediatrics;2002-03
Chinese Journal Full-text Database 10 Hits
1 ZHANG Tian-Ting et al. (The First Affiliated Hospital Hunan Medical University;Rotavirus infection in central nervous system and lung[J];Journal of Applied Clinical Pediatrics;1993-01
2 ZENG Ai-Hong. WANG Qing-Wen. HE Zheng-Xian, et al.Department of podiatrics. Third affiliated hospital of Sun Yat-Sen unixersity of medical sciences, Guang Zhou.510630 China;A clinical survey on respiratory tract infections caused by human rotavirus in children[J];JOURNAL OF APPLIED CLINICAL PEDIATRICS;1999-03
3 Chen Bin;Yao Kun;JiXiaohui;Zhou Yaoxi(Department of Microbiology,Nanjing Medical University, Nanjing 210029);The Detection of HHV-6 in Peripheral Blood Mononuclear Cells from Patients:Virus Isolation and Gene Amplification[J];VIROLOGICA SINICA;1996-02
4 Li Xinming, Li luping, Liu Jun, et al (Department of Microbiology, School of Basic Medical Sciences, China Medical University, Shenyang, 110001);Detection of HBV DNA in Mononuclear Cells from Peripheral Blood among Patients with Hepatitis B Virus Infection[J];JOURNAL OF CHINA MEDICAL UNIVERSITY;1998-06
5 Yao Yingmin, Zhao Feng, Wu Jianchun, et al. Nanfang Hospital, Guangzhou 510515;A case report of systemic infection caused by rotavirus[J];Chinexe Journal of Pediatrics;1997-07
6 Fu Wanhai, Hao Wei, Peng Yijun. Department of Pediatrics of Zhujiang Hospital. First Military University, Guangzhou 510282;Detection of rotavirus RNA using DIG labelled probe prepared by polymerase chain reaction[J];CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY;1998-01
7 XU jiazhang, JU Jiulong, YANG Zhiguo, et al. Institute of Liver Disease, 81 st Hospital of PLA, Nanjing 210002;Dynamic observation of serological and histological changes of Chinese Rhesus monkeys infected by hepatitis G virus[J];CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY;1999-01
8 Song Haijing, Gu Zhiyuan, Lu Yanping. Molecular Biology Laboratory, PLA General Hospital Beijing 100853;Detection of human cytomegalovirus DNA in brain tissues embedded in paraffin by in situ polymerase chain reaction[J];CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY;1997-06
9 Zhu Jin;Zhang Yun;Tang Jiaqi;Guo Hengbing;Wu Guanghua (Nanjing Military Medical Research Institute,Nanjing Command, Nanjing 210002);Study on Dynamic Variation of the Virus Structural Porteins and Nuclears in Peripheral Blood Mononuclear Cells from Hemorrhagic Fever with Renal Syudrome[J];CHINESE JOURNAL OF ZOONOSES;1997-03
10 CHEN Qing, SHEN Hong,YU Shouyi (Department of Epidemiology, the First Military Medical University,Guangzhou,510515);EXPERIMENT STUDY ON THE PATHOGENICITY OF ENTROTOXIGENIC ESCHERICHIA COLI IN GUINEA PIG[J];CHINESE JOURNAL OF ZOONOSES;2000-05
【Secondary References】
Chinese Journal Full-text Database 9 Hits
1 Li Ning Ou Qiaoqun~① Dai Jing~② et al.Nanfang Hospital,Guangzhou 510515,China ①Gerneral Hospital of Guangzhou Military Area Command,Guangzhou 510010,China. ②Changhai Hospital,Second Military Medical University,Shanghai 200433,China;Exploring the distribution of rotavirus receptors in human organs[J];Infectious Disease Information;2008-04
2 YAO Ying-min1, OU Qiao-qun1, CHEN Yao2 Department of Pediatrics, Nanfang Hospital1, School of Biotechnology2, Southern Medical University, Guangzhou 510515, China;Ultrastructural changes of the extraintestinal organs of newborn mice with human rotavirus infection[J];Journal of Southern Medical University;2006-09
3 YAO Ying-min1, OU Qiao-qun1, ChenYAO2 1Department of Pediatrics, Nanfang Hospital, 2College of Biotechnologies, Southern Medical University, Guangzhou 510515, China;Factors triggering extraintestinal infection of rotavirus[J];Journal of Southern Medical University;2006-10
4 Liu Lili 1 Huang Yongkun 2 Wei Qunde 2 , et al. (1. Department of Pediatrics, Beijing General Hospital of Ministry of Coal Industry, Beijing 100028; 2. Department of Pediatrics, The First Affiliated Hospital of Kunming Medical College, Kunming 650032);Detection Rotavirus Genome in the Blood of Children with Rotavirus Diarrhea by Different Kinds of PCR Tests[J];Clinical Medical Journal of China;2004-03
5 Shao Chuanda,Li Xiguang.Pediatrics Department,Yanggu County People’s Hospital. Shangdong,252300,China;The clinical significance on the detection of mycardial enzyme in child-patients with rotavirus enteritis[J];Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease;2005-04
6 NIU Zhi-min(the People's Hospital of Changyuan City,Changyuan 453400,China);Clinical significance and changes of serum myocardial enzyme spectrum in children with rotavirus enteritis[J];Journal of Xinxiang Medical College;2007-04
7 CHEN Yao1,YAO Ying-min2. (1.Postgraduate Group 1,Southern Medical University,Guangzhou 510515,China; 2. Paediatrics Department,Nanfang Hospital affiliated to Southern Medical University,Guangzhou 510515,China);Progress of the Studice on the Extraintestinal Infection of Rotavirus[J];Medical Recapitulate;2006-03
8 YAO Ying-Min,LI Ning,OU Qiao-Qun,et al.Department of Pediatrics,Southern Hospital,Southern Medical University,Guangzhou 510515,Guangdong,China.;The study of extra intestinal rotavirus infection[J];Maternal and Child Health Care of China;2008-06
9 YAO Ying-min, OU Qiao-qun, CHEN Yao (Department of Pediatrics,Nanfang Hospital,Southern Medical University, Guangzhou,510515 , China );Pathological changes of extra-intestinal infection in newborn mice infected with human rotavirus[J];Chinese Journal of Zoonoses;2006-07
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