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《ACADEMIC JOURNAL OF SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES》 1997-02
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DETECTION ON HCV RNA IN PARAFFIN EMBEDDED LIVER TISSUES BY IN SITU POLYMERASE CHAIN REACTION

Zhou Yuanping 1 Peng Wenwei 1 Yao Jilu 1 Zhang Zhigang 2 Luo Guanghan 3 Deng Zhide 1 Gu Xiuhua 1 (1 Department of Infectious Diseases, 2 Pathology, Third Affiliated Hospital, Sun Yat sen University of Medical Sciences, Guangzhou, 510630,  
In order to localize a trace of HCV RNA in tissue sections from patients with hepatocellular carcinoma (HCC), a technique of in situ polymerase chain reaction was developed and carried out in paraffin embedded sections of cancerous and pericancerous liver tissues from 17 HCC patients. The results showed that the specificity of the technique was satisfactory. The positive rates of HCV RNA in sections of cancerous and pericancerous liver tissues were 41.2% and 70.6% respectively. The positive signals mainly localized in cytoplasm. But in cancerous tissues, the positive signals in nuclei and nuclear membrane were significantly increased. These results further support the association between HCC and HCV infection and suggest the interaction between HCV gene and host genome.
【Fund】: 国家教委博士点基金 CMB资助
【CateGory Index】: R512.6
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