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Detection of Infectious Bursal Disease Viruses by in situ PCR

HUANG Guang ming,ZHANG Man fu  (College of Biological Science, China Agricultural University,Beijing 100094,China)  
The bursae were harvested from chickens which were suspected to be infected with IBDV and paraffin embedded tissue sections were prepared.IBDV were detected by in situ RT PCR after these samples treated with protease K.The results showed that eight cases presented to be positive and two negative.We tried to detect low,even single target sequence for identification of silent or mixed infection by in situ PCR which the sensitivity is very high.It is practicable to apply in situ PCR on clinical diagnosis and molecular epidemiological investigation of IBDV.
【Fund】: 国家自然科学基金!重大项目 ( 398932 90 ) ;; 国家自然科学基金资!助项目 ( 396 70 0 31)
【CateGory Index】: S858.31
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