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《Chinese Journal of Veterinary Science》 2001-03
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Detection of the Quinolone Resistance-Determining Region of the gyrA Gene of Ofloxacin Resistant Chicken Escherichia coli

LEI Lian cheng 1, HAN Wen yu 1, WANG Xing long 1, WANG Shi ruo 1,FENG Xian wei 1, JIANG Wen zheng 1,CHEN Wei 2 (1.Faculty of Animal Science and Technology, Quartermaster University of PLA, Changchun 130062, China;2.College of Animal Sc  
Thirteen ofloxacin resistant strains of chicken pathogenic E.coli were isolated from clinical samples. After plasmid extraction and purification, the quinolone resistance determining region (QRDR) of the gyrA gene was amplified by PCR with the plasmid templates. The plasmid PCR products were obtained from one strain, QRDR of the gyrA gene was also amplified by PCR from the templates of chromosomal DNA of this strain, then the PCR products were sequenced and analyzed. A expected 668 bp gyrA fragments was amplified from both plasmid DNA and chromosomal DNA of strain CEO1. The nucleotide sequences of the PCR products of plasmid DNA and chromosomal DNA showed 98.17% homology. When compared to the corresponding sequences of gyrA of E.coli from the nucleotide sequence data reported by Swanberg S L and Wang J C, 13 mutant sites were found in the nucleotide sequence of PCR product from plasmid DNA, and 3 amino acids changed; while 12 mutant sites were found in that from chromosomal DNA, and 2 amino acids changed. The results showed that the quinolone resistant gene occured both in the plasmid and chromosome of strain CE01 would be associated with quinolone resistance of strain CE01.
【Fund】: 吉林省自然科学基金!资助项目 ( 980 2 13 )
【CateGory Index】: S859.796
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