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《Chinese Journal of Veterinary Science》 2006-05
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Development of Immunochromatographic Strip Test for the Detection of Antibodyof AI

ZHANG Xi-yue,CHEN Yi-ping,FENGJuan,LI U Chun-ju,WU Xiao-dong,WANG Zhi-liang*(Animal Quarantine Institute,National Exotic Animal Disease Center,Qingdao,Shandong266032,China)  
We established ani mmunochromatographic strip(ICS) for detecting antibody of avian influenza virus(AIV).TheICS reagent was developed bylabelling antibody rabbit-anti-chicken with colloidal gold and coating Nprotein of AIVon nitrocel-lulose membrane as the capture antigen.The AI ICS was sensitive than AGP when detect their different titer of reference positiveserum.The sera fromthe chickens with NDV,IBV,IBD,EDS-76 were negative in the ICS test.Using ICS and HI test detected105 sera of chicken,the result of ICS was accordant with HI.The ICStest was proved to be rapid,briefness,specificity,and sensi-tivity for detection of antibody of AI.
【Fund】: 外来动物疫病及新发病检测平台资助项目(2004BA519A53)
【CateGory Index】: S854.43
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【Citations】
Chinese Journal Full-text Database 5 Hits
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 CHEN Shan-zhen;LIN Li-kun;WANG Zhi-hua;LIU Bo-qi;LI Zhong-sheng;YANG Jie;WANG Gui-ping;LI Qi-chang;Guangdong Haid Institute of Animal Husbandry & Veterinary Medicine;;Establishment of Colloidal Gold Immuno-chromatographic Assay for Detecting Antibodies of PRRSV and CSFV Based on Coupling Polypeptide Antigens[J];动物医学进展;2014-11
2 LI Yue;WANG Wei;ZHAO Ying-hui;ZHOU Chang-liang;ZANG Feng-xia;RAN Duo-liang;MENG Qing-wen;CHEN Hong-yan;College of Veterinary Medicine,Xinjiang Agricultural University;National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences;;Preparation of Monoclonal Antibody against Swine Influenza Virus HA by Nucleic Acid Immunization[J];中国畜牧兽医;2014-07
3 LIANG Yun-hao;CAO Chen-fu;TAO Hong;L Jian-qiang;TANG Yong;Guangdong Key Laboratory of Molecular Immunology and Antibody Engineering,Jinan University;Inspection & Quarantine Center for Animals and Plants,Shenzhen Entry-Exit Inspection and Quarantine Bureau;;Eukaryotic expression of African swine fever virus P54 protein and development of an indirect ELISA for detection of antibody against ASFV[J];中国兽医科学;2014-04
4 LI Zhong-hua;BI Ding-ren;XIAO Yun-cai;HU Si-shun;WU Ren-wei;Fujian Province Animal Disease Control Center;College of Veterinary Medicine,Huazhong Agriculture University;;Expression,Purification of Nucleoprotein of IBV and Its Application in Monitoring[J];中国生物工程杂志;2014-04
5 Liu Hongshan;Mo Jiasi;Yuan Runyu;Jiao Peirong;Luo Xiwen;College of Engineering,South China Agricultural University;College of Veterinary Medicine,South China Agricultural University;Key Laboratory of Key Technology on Agricultural Machine and Equipment,Ministry of Education,South China Agricultural University;;Design and experiment of magnetic nanobead separator for rapid detection of avian influenza virus[J];农业工程学报;2014-01
6 TIAN Shi1,DENG Guo-hua1,SHAN Ji-yan1,SHI Jian-zhong1,ZHANG Guo-quan2,CHEN Pu-cheng1, LIU Jin-xiong1,WEN Xue-xia1,JIANG Yong-ping1,CHEN Hua-lan1(1.Animal Influenza Laboratory of the Ministry Agricultural,State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China; 2.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China);Establishment of indirect ELISA method with consensus NP protein of avian influenza viruses as coated antigen[J];中国预防兽医学报;2013-06
7 YANG Hua1,YANG Fan2,YAN Yin-yin3,CAO Wei-jun2,Lü Lü2,ZHANG Rong2,LIU Hua-nan2, YANG Bo2,YANG Xiao-pu1,ZHENG Hai-xue2(1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;2.State Key Laboratory of Veterinary Etiological Biology/National Foot-and-Mouth Disease Reference Laboratory/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China; 3.The People's Government of Ganquan Town in Maiji District in Tianshui,Tianshui 741023,China);Preparation and identification of monoclonal antibody against nonstructural protein 3D of foot-and-mouth disease virus[J];中国兽医科学;2013-05
8 Ai Chunxu 1,Fan Xiaoye 1,Meng Lingwei 1,Gao Wei 2,Yuan Bao 2,Chen Jian 2,Hu Jinping 2,Ren Wenzhi 2(1 College of Animal Science and Veterinary,Jilin University,Changchun 130062;2 Laboratory Animal Center,Jilin University,Changchun 130062);Preparation of Monoclonal Antibodies against Feline Calicivirus and Their Identification of Biological Characters[J];中国农学通报;2012-29
9 WANG Qian-qian1,ZHANG Gai-ping2,WANG Xuan-nian2,3*,SONG Xing-hui2, LU Qing-xia2,WANG Shi-hong1,WANG Ai-ping1*(1.Department of Bioengineering,Zhengzhou University,Zhengzhou 450001,China; 2.Key Laboratory of Animal Immunology of the Ministry of Agriculture,Henan Provincial Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China; 3.Biotechnology Research Center,Xinxiang University,Xinxiang 453003,China);Prokaryotic Expression and Identification of P22 Antigen Peptide on VP2 Protein of Infectious Bursal Disease Virus[J];河南农业科学;2012-05
10 Junli Du1,3,Chuanxi Zhang2,Jianyu Fu1,Zhengxian Chen2,and Qiang Xiao1 1 Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 310008,Zhejiang,China 2 Institute of Insect Science,Zhejiang University,Hangzhou 310058,Zhejiang,China 3 College of Pratacultural Science,Gansu Agricultural University,Lanzhou 730070,Gansu,China;Preparation of a monoclonal antibody against polyhedrin of Ectropis obliqua nucleopolyhedrovirus[J];生物工程学报;2012-01
【Secondary Citations】
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