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《Chinese Journal of Veterinary Science》 2009-11
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The role of 1.8-kb mRNA in regulation of Marek's disease virus bi-directional promoter between pp38 and 1.8-kb mRNA

DING Jia-bo1*,LI Yan-peng2,YANG Ming3,LI Zhong-ming3,SUN Ai-jun3,Cui Zhi-zhong3(1.China Institute of Veterinary Drug Control,Beijing 100081,China;2.The Institute of Animal Science(IAS),Chinese Academy of Agricultural Sciences(CAAS),Beijing 100094,China;3.College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Taian,Shandong 271018,China)  
Marek's disease virus(MDV) contains a bi-directional promoters located between pp38 gene and 1.8-kb mRNA in the long inverted repeat region of the viral genome.The involvement of 1.8-kb mRNA in up-regulating the activity of these promoters was analyzed by transient expression of chloramphenicol acetyltransferase(CAT) reporter gene.A plasmid pP(1.8-kb-RNAi)was constructed to interference with 1.8-kb mRNA.Two CAT reporter plasmids,pP(pp38)-CAT and pP(1.8-kb)CAT,were transfected with pP(1.8-kb-RNAi) into chicken embryonic fibroblast(CEF) and CEF infected with rMd5,respectively.No CAT activity was detected in uninfected CEF as expected.The activity of the bi-directional promoter was significantly decreased in both directions when interfered by the plasmid pP(1.8-kb-RNAi.29.5% and 25.0% activity were decreased in the direction of 1.8-kb mRNA family and pp38 direction,respectively.It shows that 1.8-kb mRNA plays an important role in regulating the transcriptional activity of the bi-directional promoter.
【Fund】: 国家自然科学基金资助项目(30700596)
【CateGory Index】: S852.65
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