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《Chinese Journal of Veterinary Science》 2012-12
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Isolation and identification of endophytic fungus from the serious locoweed of China

LU Hao,LU Wei,ZHAO Bao-yu~*,CHENG Ji-ping,RONG Jie,PANG Long,SONG Yan-yan(College of Veterinary Medicine,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100,China)  
To ascertain the species,distribution and genetic development of the endophytic fungi in the serious locoweed on the west meadow of China.In this research,we collected Oxytropis.flabra,Astragalus variabilis,Oxytropis Kansuensis,Oxytropis Ochrocephala Bunge and Oxytropis Deflexa samples.Screen and purify the endophytic fungi according the different tissues of the locoweed,species and genus of endophytic fungus were identified by morphology and molecular biology.Ten fungal colony were isolated from the collected locoweed,including Didymella phacae,Fusarium proliferatum,Paecilomyces lilacinus,Ascomycota sp,Phoma glomerata,Alternaria tenuis fusarium sp,Fusarium sp,Aspergillus ustus,Schizophyllum commune.Fusarium sp and Paecilomyces lilacinus are the advantage strains,accounting for 21.1%and 37.3%of the total isolated strains,respectively.There are various species of endophytic fungi in the serious locoweed on the west meadow of China,the advantage strains in different ecological environment put up their specificity in some extent.
【Fund】: 国家自然科学基金资助项目(31072175 31201958);; 教育部高校博士点基金新教师项目(20100204120018);; 西北农林科技大学基本科研业务专项基金资助项目(QN2011108)
【CateGory Index】: S452
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