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《Chinese Journal of Veterinary Science》 2013-04
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Study on pathogenicity of H9N2 subtype avian influenza virus for oviduct of hens

WANG Jing-yu,LI Cheng-shan,CHEN Ting,WANG Li-qin,DONG Rui,ZHANG Wei-dong(College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi 712100,China)  
In order to observe H9N2 subtype influenza virus(H9N2 AIV)pathogenicity for oviduct of hens,the distribution of sialic acids α-2,3Gal receptor in oviduct of hens was investigated by using lectin histochemical staining;H9N2 AIV was inoculated to observe histopathological changes of oviduct;primary cells of oviduct magnum epithelial were cultured with collagenase I digestion,pathological changes were observed after inoculating H9N2 AIV.The result showed that SAα-2,3Gal receptors were expressed in chicken oviduct except infundibulum.Histopathological changes showed epithelial villus fell off or epithelial cell necrosis in magnum and broad hyperemia,hemorrhage in tissue of shell gland;primary cells show no obvious histopathological changes at 72 h after inoculated H9N2 AIV,but the viral titer achieved by 3log2.From the above results,H9N2 AIV can encroach on oviduct of hens,and cause histopathological changes.
【Fund】: 国家自然科学基金资助项目(31272577);; 陕西省科技统筹创新工程计划资助项目(2011KT-DZ02-01)
【CateGory Index】: S858.31
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