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Monoclonal Antibodies Recognizing Serotype Ⅰ Specific and Group Common Epitypes on Glucoprotein B Molecule of Marek′s Disease Virus

Lu Changming Cui Zhizhong Qin Aijian (Agriculture College of Yangzhou University, Yangzhou, Jiangsu 225009) Lee L F (Avian Disease and Oncology Laboratory, USDA)  
To prepare monoclonal antibody (McAb) against glycoprotein B (gB) of Marek′s disease virus (MDV), the mice were immunized by the Sf9 cells nfected with gB expressing recombinant baculovirus and hybridoma cells were screened by indirect fluorescent antibody (IFA) technique and ELISA with serotype Ⅰ MDV GA strain infected chicken embryo fibroblast. In IFA and immunoprecipitation test, McAb BA4 reacted only to serotype Ⅰ MDV CEF (including CVI988 vaccine strains) and McAb BD8 gave positive reaction to all serotypes of MDV. Immunoprecipitation of 35 S methionine labelled MDV CEF lysates indicated that both McAb BA4 and BD8 could recognize MDV gB of 100 000, 60 000 and 49 000.
【Fund】: 国家自然科学基金重点项目
【CateGory Index】: S852.3
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