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《Chinese Journal of Veterinary Drug》 2002-06
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Cloning of Calcitonin Gene in Lactobacillus

HE Zhao-qing, QIN Ze-rong, XU Bin-rui, WANG Chun-feng, ZHANG Li, LIU Shang-gao(College of Veterinary Medicine, China Agricultural University, Beijing 100094; China )  
Calcitonin gene was synthesized according to the sequence of salmon Calcitonin gene. The gene andunantibitic expressive vector pW425t were both digested by SacI and KpnI. Two parts were connected by T4 DNAligase. The recombined plasmid pWCT proved containing Calcitonin gene by restriction enzyme digestion andPCR. 100bp fragment was amplified by PCR from the recombinant plasmid pWCT using the primers P1/P2. Therecombinant plasmid pWCT was then cloned into Lactobacillus by electric-translation.The sequence of the Calci-tonin gene was determined. Compared with those of salmon Calcitonin, the identities of predicted amino acids are100%. The recombined Lactobacillus will be used to express salmon Calcitonin, and be used to prevent and treatCaged Layer Osteoporosis.
【Fund】： 本研究由北京普仁生态技术有限公司资助
【CateGory Index】： Q785
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