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《Chinese Journal of Veterinary Medicine》 2014-02
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Isolation identification and sequencing VP1 gene of avian encephalomyelitis virus YL strain

HAO Hua-fang;ZHANG Shu-xia;YANG Tao;YANG Zeng-qi;WANG Xing-long;DU En-qi;DANG Ru-yi;WANG Jing-yu;College of Veterinary Medicine,Northwest A&F University;  
In order to clarify the reason of a sudden decrease in production,a low hatchability,and an increasing number of deadand weak chicks on a chicken farm in Yangling Shaanxi province. The pathogen was identified by collecting the brain tissue continuous passage on SPF chicken embryos,Agar Gel precipitin(AGP) test,experimental infection,cloning and sequencing analysis of VP1 gene. The results showed that specific precipitation lines formed between the isolation YL and AEV standard positive serum in AGP assay; the chicks exhibited tremors,ataxia,edema and softening of the brain in experimental infection; sequencing analysis results showed that the full length sequence of VP1 gene of YL strain consisted of 810 nucleotides,coding 270 amino acid residues and it shared 92.0 %~99.8 % nucleic acid identities and 89.3 %~99.3 % amino acid identities with the AEV reference strain;evolution analysis indicated that YL strain was closely related to 1143,L2Z,SX strain and distantly related to VR,HB,SD,NH937 strain. The results pointed out that the isolation was avian encephalomyelitis virus,and it showed a certain degree of difference in evolution compared with AEV known strains.
【Fund】: 陕西省科技统筹创新工程计划项目(2011KTDZ02-01);; 西北农林科技大学博士启动基金(01140401)
【CateGory Index】: S852.65
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1 LIU Li-hua1,MA Xue-en2,ZHAO Zhen-hua2 (1.School of Chemical Engineering,Inner Mongolia University of Technology,Hohhot 010051,China;2.School of Animal Science and Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China);FUSION EXPRESSION AND ACTIVITY ASSAY OF VP_1 CAPSID PROTEIN GENE OF AVIAN ENCEPHALOMYCLITIS VIRUS NH937 STRAIN ISOLATED IN INNIER MONGOLIA[J];Journal of Inner Mongolia University of Technology(Natural Science);2007-03
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